Phytoestrogens reduce bone loss and bone resorption in oophorectomized rats
Phytoestrogens reduce bone loss and bone resorption in oophorectomized rats
复制标题
DOI：
10.1093/jn/127.9.1795
复制
发表时间：
1997-09-01
影响因子：
4.2
通讯作者：
Prince, RL
中科院分区：
文献类型：
--
作者：
Draper, CR;Edel, MJ;Prince, RL
为了研究植物雌激素在绝经后骨质流失中的潜在作用，在三项研究中使用了卵巢切除（OOX）大鼠模型，以研究植物雌激素香豆雌酚、玉米赤霉醇和一种雌激素酮混合物对雌激素依赖性骨质流失的影响。在香豆雌酚和玉米赤霉醇的研究中，将大鼠分配到对照组、植物雌激素处理组（1.5 μ mol香豆雌酚或3.1 mmol玉米赤霉醇，每周两次，肌肉注射）或香豆雌酚研究中的雌激素处理组（28.1 nmol，肌肉注射）。在研究中，将大鼠分配到对照组、雌激素治疗组或每周接受131.25 mg植物雌激素的治疗组，所述植物雌激素掺入未纯化的大鼠饮食中。在基线和卵巢切除术后6周测量全身、脊柱和股骨的骨矿物质密度。在香豆雌酚研究中，采集了血液和尿液样本。与对照组相比，接受香豆雌酚和玉米赤霉醇的大鼠在所有测量部位的骨丢失均显著减少。雌激素治疗组在脊柱和整体测量中的骨密度显著高于对照组和香豆雌酚治疗组。治疗1周后，香豆雌酚减少尿钙排泄和骨吸收标志物吡啶啉和脱氧吡啶啉。口服植物雌激素对卵巢切除大鼠没有影响，包括所用剂量的骨丢失。因此，香豆雌酚和玉米赤霉醇在预防骨丢失方面的生物活性首次在公认的绝经后骨丢失模型中得到证实。
To examine a potential role for phytoestrogens in postmenopausal bone loss, the oophorectomized (OOX) rat model has been used in three studies to investigate the effects of the phytoestrogens coumestrol, zearalanol and a mixture of isoflavones on estrogen-dependent bone loss. In the studies of coumestrol and zearalanol, the rats were allocated to a control group, a phytoestrogen-treated group (1.5 mu mol coumestrol or 3.1 mmol zearalanol twice per week, intramuscular) or, in the coumestrol study, an estrogen-treated group (28.1 nmol, intramuscular). In the isoflavone study, the rats were allocated to a control group, an estrogen treated group or a treatment group that received 131.25 mg of phytoestrogens per week incorporated into the nonpurified rat diet. Bone mineral density was measured globally and at the spine and femur at base line and 6 wk post-oophorectomy. In the coumestrol study, blood and urine samples were collected. Compared with the control group, rats receiving coumestrol and zearalanol had significantly reduced bone loss at all sites measured. The estrogen-treated group had significantly greater bone density than the control and the coumestrol-treated groups in the spine and global measurements. Coumestrol reduced urine calcium excretion and the bone resorption markers pyridinoline and deoxypyridinoline after 1 wk of treatment. Oral isoflavone phytoestrogens had no effect on oophorectomized rats including bone loss at the dose used. Thus, for the first time, the bioactivity of coumestrol and zearalanol in preventing bone loss has been demonstrated in a well-recognized model of postmenopausal bone loss.