Comparative study of the intestinal mucus barrier in normal and inflamed colon
Comparative study of the intestinal mucus barrier in normal and inflamed colon
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DOI：
10.1136/gut.2006.098160
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发表时间：
2007-03-01
期刊：
影响因子：
24.5
通讯作者：
Doerffel, Yvonne
中科院分区：
文献类型：
--
作者：
Swidsinski, Alexander;Loening-Baucke, Vera;Doerffel, Yvonne
目的：为了研究粘液的作用，在空间分离的肠道细菌从mucosal.Patients和方法：粘液屏障特性进行了评价，使用组织学材料从清洗结肠活检，结肠制备灌肠剂和材料从未经处理的阑尾固定与非水卡诺解决方案。细菌进行了评价，使用荧光原位杂交，与细菌16 S RNA探针和相关的高碘酸希夫阿辛蓝染色。对照组（n = 20），自限性结肠炎（SLC; n = 20），溃疡性结肠炎（n = 20）和60例随机选择的appendics.Results：粘液层下的粘膜表面无细菌>= 80%的正常阑尾和对照组的活检组织。随着炎症严重程度的增加，粘液层的厚度及其扩散程度降低;上皮表面显示细菌粘附、上皮组织缺损以及细菌和白细胞的深层粘膜浸润。在溃疡性结肠炎、SLC和急性阑尾炎患者的所有活检标本中，均在粘液中发现细菌和白细胞。粘液内的细菌浓度呈负相关的白细胞numbers of the leukocytes.Conclusions：大肠粘液层有效地防止接触高度集中的腔细菌和上皮细胞在所有部分的正常结肠。结肠炎症总是伴随着粘液屏障的破坏。虽然炎症反应逐渐减少粘液和粪便中的细菌数量，但炎症本身不能阻止细菌迁移、粘附和侵入粘膜。
Aim: To study the role of mucus in the spatial separation of intestinal bacteria from mucosa.Patients and methods: Mucus barrier characteristics were evaluated using histological material obtained by biopsy from purged colon, colon prepared with enema and material from untreated appendices fixed with non-aqueous Carnoy solution. Bacteria were evaluated using fluorescence in situ hybridization, with bacterial 16S RNA probes and related to the periodic acid Schiff alcian blue stain. Biopsies from controls (n = 20), patients with self-limiting colitis (SLC; n = 20), ulcerative colitis ( n = 20) and 60 randomly selected appendices were investigated.Results: The mucosal surface beneath the mucus layer was free of bacteria in >= 80% of the normal appendices and biopsies from controls. The thickness of the mucus layer and its spread decreased with increasing severity of the inflammation; the epithelial surface showed bacterial adherence, epithelial tissue defects and deep mucosal infiltration with bacteria and leucocytes. Bacteria and leucocytes were found within mucus in all biopsy specimens from patients with ulcerative colitis, SLC, and acute appendicitis. The concentration of bacteria within mucus was inversely correlated to the numbers of leucocytes.Conclusions: The large bowel mucus layer effectively prevents contact between the highly concentrated luminal bacteria and the epithelial cells in all parts of the normal colon. Colonic inflammation is always accompanied by breaks in the mucus barrier. Although the inflammatory response gradually reduces the number of bacteria in mucus and faeces, the inflammation itself is not capable of preventing bacterial migration, adherence to and invasion of the mucosa.