Modern Pathology Methods for Neural Investigations
Modern Pathology Methods for Neural Investigations
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DOI：
10.1177/0192623310394213
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发表时间：
2011-01-01
影响因子：
1.5
通讯作者：
Little, Peter B.
中科院分区：
文献类型：
--
作者：
Hale, Sarah L.;Andrews-Jones, Lydia;Little, Peter B.
2010 年毒理学病理学会 (STP) 和国际毒理学病理学家学会联合会 (IFSTP) 联合研讨会上的本次会议探讨了评估异生物质神经毒理学潜力的现代神经病理学方法。回顾了最佳制备和评估中枢和外周神经组织同时最大限度地减少伪影的传统技术，并提出了在常规（即一般毒性）研究和增强（即专门神经毒性）研究期间评估神经系统的最佳方案。体视学被引入作为检查毒物对神经细胞数量可能影响的最合适的方法。神经科学家专家小组（解剖学家和病理学家）和观众就大脑采样进行了集中讨论，讨论了充分采样与分析的成本和时间有效性之间的适当平衡。关于切片方向（两侧的冠状切片与从对侧进行数个单侧半切片的旁矢状纵向切片）尚未达成共识，但大多数小组成员赞成在常规毒性研究中至少采样 8 个切片（或大约是当前补充的两倍至三倍）。
This session at the 2010 joint symposium of the Society of Toxicologic Pathology (STP) and the International Federation of Societies of Toxicologic Pathologists (IFSTP) explored modern neuropathology methods for assessing the neurotoxicologic potential of xenobiotics. Conventional techniques to optimally prepare and evaluate the central and peripheral neural tissues while minimizing artifact were reviewed, and optimal schemes were set forth for evaluation of the nervous system during both routine (i.e., general toxicity) studies and enhanced (i.e., specialized neurotoxicity) studies. Stereology was introduced as the most appropriate means of examining the possible impact of toxicants on neural cell numbers. A focused discussion on brain sampling took place among a panel of expert neuroscientists (anatomists and pathologists) and the audience regarding the proper balance between sufficient sampling and cost-and time-effectiveness of the analysis. No consensus was reached on section orientation (coronal sections of both sides vs. a parasagittal longitudinal section with several unilateral hemisections from the contralateral side), but most panelists favored sampling at least 8 sections (or approximately double to triple the current complement) in routine toxicity studies.