Purification and characterization of a protease from Pseudomonas aeruginosa grown in cutting oil
Purification and characterization of a protease from Pseudomonas aeruginosa grown in cutting oil
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DOI：
10.1016/s1389-1723(04)00258-0
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发表时间：
2004-09-01
影响因子：
2.8
通讯作者：
Fujiwara, N
中科院分区：
文献类型：
--
作者：
Karadzic, I;Masui, A;Fujiwara, N
铜绿假单胞菌 san-ai 菌株是从用于工业金属加工过程中冷却和润滑的水溶性切削油中分离出来的。该菌株在表面活性剂和矿物油的高碱性（pH 10）混合物中生长，产生细胞外蛋白水解酶。我们已经纯化并表征了这种 18 kDa 蛋白酶。铜绿假单胞菌蛋白酶在 pH 9.0 和 60 摄氏度下发挥最佳作用。另外，它是一种含锌金属酶，其单体结构含有至少一个二硫键。由于该酶在有机溶剂存在下稳定，因此适用于肽合成。此外，铜绿假单胞菌蛋白酶可用于智能药物输送系统（DDS），该系统专为金属工业应用而设计，用于防止切削油腐败。
The Pseudomonas aeruginosa san-ai strain was isolated from water-soluble cutting oil used for cooling and lubrication during industrial metal-working processes. This strain, which is grown in a high alkaline (pH 10) mixture of surfactants and mineral oil, produces an extracellular proteolytic enzyme. We have purified and characterized this 18 kDa protease. The P aeruginosa san-ai protease functions optimally at pH 9.0 and 60degreesC. Additionally, it is a Zn-containing metalloenzyme, and its monomeric structure contains at least one disulfide bond. Because the enzyme is stable in the presence of organic solvents, it is suitable for peptide synthesis. Furthermore, the P aeruginosa san-ai protease could be used in an intelligent drug delivery system (DDS) designed for applications in the metal industry for prevention of putrefaction of cutting oil.