The sporulation of the green alga Ulva prolifera is controlled by changes in photosynthetic electron transport chain.
The sporulation of the green alga Ulva prolifera is controlled by changes in photosynthetic electron transport chain.
复制标题
绿藻石莼的孢子形成是由光合电子传递链的变化控制的
DOI：
10.1038/srep24923
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发表时间：
2016-04-22
影响因子：
4.6
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Wang H;Lin A;Gu W;Huan L;Gao S;Wang G
产孢和孢子释放是藻类和陆生植物生命周期的重要阶段。本研究以研究孢子形成和孢子释放的理想生物增殖Ulva prolifera为实验材料。本实验采用显微观察、光合抑制剂处理、碳捕获限制和蛋白质质谱法测定光合参数。循环电子传递（CEF）在孢子囊形成时增强。二溴百里醌（DBMIB）对产孢的抑制作用在产孢过程中始终很强，而3-（3′，4′-二氯苯基）-1,1-二甲基脲（DCMU）的抑制作用随着产孢的进行而不断减弱。由于CO2获取的限制导致的光合作用的变化可以刺激孢子形成。定量蛋白质分析表明，在孢子形成过程中，参与碳固定的酶，包括RUBISCO和丙酮酸正磷酸二激酶，减少，而参与孢子形成的蛋白质，包括微管蛋白和中心蛋白，增加。综上所述，绿藻胞内循环电子流（CEF）的增强和质体醌池的氧化对孢子囊的形成起着至关重要的作用，光合电子传递链的变化对绿藻的产孢有重要影响。
Sporulation and spore release are essential phases of the life cycle in algae and land plants. Ulva prolifera, which is an ideal organism for studying sporulation and spore release, was used as the experimental material in the present study. The determination of photosynthetic parameters, combined with microscopic observation, treatment with photosynthetic inhibitors, limitation of carbon acquisition, and protein mass spectrometry, was employed in this experiment. Cycle electron transport (CEF) was found enhanced at the onset of sporangia formation. The inhibition effect of dibromothymoquinone (DBMIB) towards sporulation was always strong during the sporulation process whereas the inhibition effect of 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU) was continuously declined accompanied with the progress of sporulation. The changes of photosynthesis resulted from the limitation of CO2 acquisition could stimulate sporulation onset. Quantitative protein analysis showed that enzymes involved in carbon fixation, including RUBISCO and pyruvate orthophosphate dikinase, declined during sporogenesis, while proteins involved in sporulation, including tubulin and centrin, increased. These results suggest that enhanced cyclic electron flow (CEF) and oxidation of the plastoquinone pool are essential for sporangia formation onset, and changes in photosynthetic electron transport chain have significant impacts on sporulation of the green algae.