Nature of telomere dimers and chromosome looping in human spermatozoa.
Nature of telomere dimers and chromosome looping in human spermatozoa.
复制标题
人类精子中端粒二聚体和染色体环的性质。
DOI：
10.1007/s10577-005-5513-1
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Zalensky,AndreiO
中科院分区：
文献类型：
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作者：
Solov'eva,Lyudmila;Svetlova,Maria;Bodinski,Dawn;Zalensky,AndreiO
人类精子细胞中特定且组织良好的染色体结构是由着丝粒和端粒之间的显著相互作用支持的。端粒与端粒的相互作用导致端粒二聚体位于细胞核外围。目前尚不清楚精子端粒二聚体的组成是随机的还是特定的。我们现在报告端粒二聚体是由每条染色体两端之间的特定相互作用产生的。FISH使用对亚端粒DNA探针，这些探针对应于七条人类染色体的小臂和长臂，证明一条染色体的亚端粒是聚集在一起的。统计分析证实，端粒关联不可能是DNA序列随机接近的结果。因此，人类精核中的染色体采用环状构象。这种高阶染色体结构很可能是导致合子形成的早期受精事件中染色体撤回/去凝聚所必需的。
Specific and well-organized chromosome architecture in human sperm cells is supported by the prominent interactions between centromeres and between telomeres. The telomere-telomere interactions result in telomere dimers that are positioned at the nuclear periphery. It is unknown whether composition of sperm telomere dimers is random or specific. We now report that telomere dimers result from specific interactions between the two ends of each chromosome. FISH using pairs of subtelomeric DNA probes that correspond to the small and long arms of seven human chromosomes demonstrates that subtelomeres of one chromosome are brought together. Statistical analysis confirmed that telomere associations could not result from the random proximity of DNA sequences. Therefore, chromosomes in human sperm nuclei adopt a looped conformation. This higher-order chromosome structure is most likely required for chromosome withdrawal/decondensation during the early fertilization events leading to zygote formation.