The estimation of PAH bioavailability in contaminated sediments using hydroxypropyl-β-cyclodextrin and Triton X-100 extraction techniques
The estimation of PAH bioavailability in contaminated sediments using hydroxypropyl-β-cyclodextrin and Triton X-100 extraction techniques
复制标题
DOI：
10.1016/s0045-6535(01)00199-0
复制
发表时间：
2002-03-01
期刊：
影响因子：
8.8
通讯作者：
Rulkens, W
中科院分区：
文献类型：
--
作者：
Cuypers, C;Pancras, T;Rulkens, W
研究了环糊精和表面活性剂能否用于预测污染沉积物中多环芳烃（PAH）的生物有效性。用羟丙基-β-环糊精（HPCD）和Triton X-100水溶液提取两个沉积物样品。比较了萃取法、生物降解法和固相萃取法对多环芳烃的去除效果。后两种方法被用来作为参考方法，以确定哪些部分的多环芳烃可以被生物降解，以及在何种程度上生物降解是由生物利用度的限制。结果表明，HPCD提取后固相萃取和去除主要是容易生物可利用的多环芳烃，而Triton X-100提取容易和生物可利用性差的多环芳烃。在生物降解实验中，HPCD对多环芳烃的降解没有影响，而Triton X-100对低分子量多环芳烃的降解有促进作用。结果表明，HPCD提取法可为PAH生物利用度的预测提供一种较好的方法。TritonX-100萃取法不适合于PAH生物利用度的预测。(C)2002爱思唯尔科技有限公司。保留所有权利。
A study was conducted to investigate whether cyclodextrins and surfactants can be used to predict polycyclic aromatic hydrocarbon (PAH) bioavailability in contaminated sediments. Two sediment samples were extracted with aqueous solutions of hydroxypropyl-p-cyclodextrin (HPCD) and Triton X-100. PAH removal during extraction was compared with PAH removal during biodegradation and solid-phase extraction. The latter two methods were used as reference methods to establish which part of the PAHs could be biodegraded and to what extent biodegradation was governed by bioavailability limitations. It was demonstrated that HPCD extraction followed solid-phase extraction and removed primarily readily bioavailable PAHs, while Triton X-100 extracted both readily and poorly bioavailable PAHs. Moreover, HPCD did not affect the degradation of PAHs in biodegradation experiments, while Triton X-100 enhanced the degradation of low molecular weight PAHs. It was concluded that HPCD extraction may provide a good method for the prediction of PAH bioavailability. Triton X-100 extraction is unfit for the prediction of PAH bioavailability. (C) 2002 Elsevier Science Ltd. All rights reserved.