Neuregulin-1β regulates outgrowth of neurites and migration of neurofilament 200 neurons from dorsal root ganglial explants in vitro
Neuregulin-1β regulates outgrowth of neurites and migration of neurofilament 200 neurons from dorsal root ganglial explants in vitro
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DOI：
10.1016/j.peptides.2011.04.005
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发表时间：
2011-06-01
期刊：
影响因子：
3
通讯作者：
Li, Zhenzhong
中科院分区：
文献类型：
--
作者：
Liu, Zhen;Gao, Wei;Li, Zhenzhong
神经调节蛋白-1 β（NRG-1 β）信号在神经元中具有多种功能。为了研究NRG-1 β对体外培养的神经元突起生长和迁移的影响，建立了器官型背根神经节（DRG）神经元培养模型。在存在（5 nmol/L、10 nmol/L、20 nmol/L）或不存在NRG-1 β的情况下，使用该培养模型评价神经突生长和神经元迁移。神经丝200（NF-200）免疫反应（IR）神经元被确定为迁移神经元。在NRG-1 β存在的情况下，从DRG外植体延伸的神经纤维束的数量显著增加（5 nmol/L，23.0 ± 2.2，P < 0.05; 10 nmol/L，27.0 ± 2.7，P < 0.001; 20 nmol/L，30.8 +/- 3.7，P < 0.001），与无NRG-1 β时（19.0 +/- 2.2）相比。在NRG-1 β存在下，从DRG外植体迁移的神经元数量显著增加（5 nmol/L，39.6 ± 5.0，P < 0.05; 10 nmol/L，54.6 ± 6.7，P < 0.001; 20 nmol/L，62.2 +/- 5.7，P < 0.001），与无NRG-1 β时（31.6 +/- 4.0）相比。此外，神经纤维束的数量和迁移的NF-200-IR神经元的数量的增加是剂量依赖性的NRG-1 β添加。本研究中的数据表明，NRG-1 β在体外促进DRG外植体的神经突起生长和神经元迁移。(C)2011 Elsevier Inc. All rights reserved.
Neuregulin-1 beta (NRG-1 beta) signaling has multiple functions in neurons. To assess NRG-1 beta on neurite outgrowth and neuronal migration in vitro, organotypic dorsal root ganglion (DRG) neuronal culture model was established. Neurite outgrowth and neuronal migration were evaluated using this culture model in the presence (5 nmol/L, 10 nmol/L, 20 nmol/L) or absence of NRG-1 beta. Neurofilament 200 (NF-200)-immunoreactive (IR) neurons were determined as the migrating neurons. The number of nerve fiber bundles extended from DRG explant increased significantly in the presence of NRG-1 beta (5 nmol/L, 23.0 +/- 2.2, P < 0.05; 10 nmol/L, 27.0 +/- 2.7, P < 0.001; 20 nmol/L, 30.8 +/- 3.7, P < 0.001) as compared with that in the absence of NRG-1 beta (19.0 +/- 2.2). The number of neurons migrating from DRG explants increased significantly in the presence of NRG-1 beta (5 nmol/L, 39.6 +/- 5.0, P < 0.05; 10 nmol/L, 54.6 +/- 6.7, P < 0.001; 20 nmol/L, 62.2 +/- 5.7, P < 0.001) as compared with that in the absence of NRG-1 beta (31.6 +/- 4.0). Moreover, the increase of the number of nerve fiber bundles and the number of migrating NF-200-IR neurons was dose-dependent for NRG-1 beta addition. The data in this study imply that NRG-1 beta promotes neurite outgrowth and neuronal migration from DRG explants in vitro. (C) 2011 Elsevier Inc. All rights reserved.