PATHOVARS OF XANTHOMONAS-CAMPESTRIS ARE DISTINGUISHABLE BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM
PATHOVARS OF XANTHOMONAS-CAMPESTRIS ARE DISTINGUISHABLE BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM
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DOI：
10.1099/00207713-37-3-214
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发表时间：
1987-07-01
期刊：
影响因子：
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通讯作者：
GABRIEL, DW
中科院分区：
文献类型：
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作者：
LAZO, GR;ROFFEY, R;GABRIEL, DW
克隆的脱氧核糖核酸（DNA）片段，来源于野油菜黄单胞菌致病变种（Xanthomonascampestrispv.）的佛罗里达分离株的粘粒文库。citri 3041对93株X. campestris，共26个致病变种。DNA克隆进行放射性标记，并与不同菌株的X.用限制性内切酶消化的油菜。对基因组DNA探测的DNA克隆的放射自显影显示杂交谱，这似乎是高度保守和独特的每个致病变种测试。作为一个种，其种群结构是X. campestris基本上呈无性系。变异性似乎取决于所使用的DNA探针;一些探针代表高度变异的DNA位点，而另一些则代表在物种水平上高度保守的DNA位点。通过使用多种DNA探针或用不同的限制性内切酶消化基因组DNA，我们能够区分所有的X。被描述为属于特定致病变种的野油菜。植物致病性试验也证实了致病变种之间的差异。一些分离自植物的病原菌株以前未被描述为对X. campestris可以被归类为与先前描述的致病变种相关。
Cloned deoxyribonucleic acid (DNA) fragments, derived from a cosmid library of a Florida isolate of Xanthomonas campestris pv. citri 3041 were used to detect restriction fragment-length polymorphism among 93 strains of X. campestris, which make up 26 pathovars. DNA clones were radiolabeled and hybridized against Southern blot transfers of genomic DNAs of different strains of X. campestris digested with restriction endonucleases. Autoradiographs for DNA clones probed against genomic DNA revealed hybridization profiles which appeared to be highly conserved and unique for each pathovar tested. As a species, the population structure of X. campestris appeared basically clonal. Variability appeared to depend on the DNA probe used; some probes represented DNA loci which were highly variable, and some represented DNA loci which were highly conserved at the species level. By using more than one DNA probe, or by digesting the genomic DNAs with different restriction endonucleases, we were able to differentiate all of the strains of X. campestris described as belonging to a given pathovar. Differences among the pathovars were also confirmed by pathogenicity experiments on plants. Some phytopathogenic strains isolated from plants not previously described as susceptible to X. campestris could be grouped as being related to previously described pathovars.