Role of mast cells in acupuncture effect: A pilot study
Role of mast cells in acupuncture effect: A pilot study
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DOI：
10.1016/j.explore.2008.02.002
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发表时间：
2008-05-01
影响因子：
2.4
通讯作者：
Gu, Quanbao
中科院分区：
文献类型：
--
作者：
Zhang, Di;Ding, Guanghong;Gu, Quanbao
为了更好地了解针灸的治疗效果，需要解决有关潜在机制的问题。在此，我们描述了针刺足三里对大鼠镇痛的影响。刺激ST36后的镇痛作用比刺激穴位附近的假穴位后更明显。同时测定组织切片中针刺穴位及邻近穴位的肥大细胞密度，以及刺激前后的脱颗粒程度。我们发现大鼠ST36的肥大细胞密度高于邻近假手术点的肥大细胞密度。此外，针刺可引起肥大细胞脱颗粒显著增加。穴位经铬酸二钠预处理后，既可对抗脱粒现象，又可降低针刺的镇痛效果。我们对穴位组织中肥大细胞脱颗粒的抑制实验证实了肥大细胞在针刺效应中的可能作用。
To better understand the therapeutic effectiveness of acupuncture, questions about the underlying mechanisms need to be addressed. Here we describe the impact of manual stimulation by an acupuncture needle of zusanli (stomach 36 [ST36]) on analgesia in rats. The analgesic effect was more pronounced after stimulation of ST36 than after stimulation of a sham point near the acupuncture point. At the same time, we determined in tissue slices the density of mast cells in the acupuncture points and nearby points, as well as the degree of degranulation before and after stimulation. We found that the density of mast cells from the ST36 of rats was higher than that from a nearby sham point. In addition, acupuncture resulted in a remarkable increase in degranulation of the mast cells. Pretreatment of the acupuncture point with disodium chromoglycate not only counteracted the phenomenon of degranulation but also reduced analgesic effect of acupuncture. Our experiments on inhibition of degranulation of mast cells in tissue from acupuncture points demonstrates the possible role of mast cells in acupuncture effects.