The H2O2 stimulon in Saccharomyces cerevisiae
The H2O2 stimulon in Saccharomyces cerevisiae
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DOI：
10.1074/jbc.273.35.22480
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发表时间：
1998-08-28
影响因子：
4.8
通讯作者：
Labarre, J
中科院分区：
文献类型：
--
作者：
Godon, C;Lagniel, G;Labarre, J
通过比较细胞总蛋白的二维凝胶电泳分析了酵母对H2O2适应性胁迫的基因表达变化。H2O2刺激了至少115种蛋白质的合成，而其他52种蛋白质则受到抑制。我们已经确定了71个受刺激的目标和44个受抑制的目标。分析了H2O2基因组响应的动力学和剂量响应参数。这些蛋白质的鉴定和它们在特定细胞过程中的映射，为酵母细胞适应氧化应激的方式提供了一幅清晰的画面。正如预期的那样，h2o2反应靶标包括大量的热休克蛋白和具有活性氧中间体清除活性的蛋白。暴露于H2O2也会导致蛋白质生物合成过程的减慢和蛋白质降解途径的刺激。最后，本研究得出的最显著的结果是暴露于H2O2几分钟后碳水化合物代谢的重置。碳水化合物的通量以糖酵解为代价，重新导向NADPH的再生。本研究首次在真核生物中对h2o2诱导的刺激子进行全基因组鉴定。
The changes in gene expression underlying the yeast adaptive stress response to H2O2 were analyzed by comparative two-dimensional gel electrophoresis of total cell proteins. The synthesis of at least 115 proteins is stimulated by H2O2, whereas 52 other proteins are repressed by this treatment. We have identified 71 of the stimulated and 44 of the repressed targets. The kinetics and dose-response parameters of the H2O2, genomic response were also analyzed. Identification of these proteins and their mapping into specific cellular processes give a distinct picture of the way in which yeast cells adapt to oxidative stress. As expected, H2O2-responsive targets include an important number of heat shock proteins and proteins with reactive oxygen intermediate scavenging activities. Exposure to H2O2 also results in a slowdown of protein biosynthetic processes and a stimulation of protein degradation pathways. Finally, the most remarkable result inferred from this study is the resetting of carbohydrate metabolism minutes after the exposure to H2O2. Carbohydrate fluxes are redirected to the regeneration of NADPH at the expense of glycolysis. This study represents the first genome-wide characterization of a H2O2-inducible stimulon in a eukaryote.