Purification and culture of astrocytes.
Purification and culture of astrocytes.
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DOI：
10.1101/pdb.top070912
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发表时间：
2013-06-01
影响因子：
--
通讯作者：
Foo, Lynette C
中科院分区：
文献类型：
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作者：
Foo, Lynette C
多年来，神经胶质细胞相互作用的研究一直依赖于使用星形胶质细胞从新生啮齿动物大脑分离的未成熟前体细胞的扩展培养。虽然在这些选择性细胞存活条件下培养的星形胶质细胞已经成为理解星形胶质细胞行为的重要工具，但它们不一定反映成熟星形胶质细胞的行为和功能。我们已经开发了急性，前瞻性的分离和培养的成熟星形胶质细胞从啮齿动物的大脑中的无血清，确定的培养基的方法。这些基于免疫印迹的方法促进了星形胶质细胞生物学和功能的研究。
For years, studies of neural-glial interactions have relied on the use of astrocytes derived from the extended culture of immature precursor cells isolated from the neonatal rodent brain. Although the astrocytes cultured under these selective cell survival conditions have been important tools for understanding astrocyte behavior, they do not necessarily reflect the behavior and function of mature astrocytes. We have developed methods for acute, prospective isolation and culture of mature astrocytes from rodent brains in a serum-free, defined medium. These immunopanning-based methods facilitate the study of astrocyte biology and function.