Enhanced depressor response to oxide synthase gene transfer endothelial nitric into the nucleus tractus Solitarii of spontaneously hypertensive rats
Enhanced depressor response to oxide synthase gene transfer endothelial nitric into the nucleus tractus Solitarii of spontaneously hypertensive rats
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DOI：
10.1291/hypres.26.325
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发表时间：
2003-04-01
影响因子：
5.4
通讯作者：
Takeshita, A
中科院分区：
文献类型：
--
作者：
Hirooka, Y;Sakai, K;Takeshita, A
以前，我们证明了内皮型一氧化氮合酶（eNOS）基因转移到孤束核（NTS）降低血压，心率和交感神经活动在清醒血压正常的Wistar-Kyoto大鼠（WKY）。为了研究eNOS在孤束核中的过表达是否对自发性高血压大鼠（SHR）和WKY的血压和心率产生不同的影响，我们将编码eNOS（AdeNOS）或β-半乳糖苷酶（Ad beta gal）的腺病毒载体在体内转染到SHR和WKY的孤束核中。eNOS蛋白的Western印迹分析证实了NTS中eNOS的局部表达，并且表达的幅度在SHR和WKY之间没有差异。在基因转移前和基因转移后7天，通过使用无线电遥测系统在清醒状态下监测血压和心率。在AdeNOS转染的SHR和WKY中，收缩压（SBP）和心率在第7天降低。然而，AdeNOS转染的SHR的SBP下降幅度大于AdeNOS转染的WKY（-24.1+/-2.9 vs. -15.9+/-2.1 mmHg，p
Previously, we demonstrated that endothelial nitric oxide synthase (eNOS) gene transfer into the nucleus tractus solitarii (NTS) decreased blood pressure, heart rate and sympathetic nerve activity in conscious normotensive Wistar-Kyoto rats (WKY). In order to determine whether overexpression of eNOS in the NTS causes different effects on blood pressure and heart rate between spontaneously hypertensive rats (SHR) and WKY, we transfected adenovirus vectors encoding either eNOS (AdeNOS) or beta-galactosidase (Ad beta gal) into the NTS of SHR and WKY in vivo. The local expression of eNOS in the NTS was confirmed by Western blot analysis for eNOS protein, and the magnitude of expression did not differ between SHR and WKY. Blood pressure and heart rate were monitored by the use of a radio-telemetry system in a conscious state before and 7 days after the gene transfer. Systolic blood pressure (SBP) and heart rate decreased on day 7 in both AdeNOS-transfected SHR and WKY. However, the magnitude of decreases in SBP of AdeNOS-transfected SHR was greater than that of AdeNOS-transfected WKY (-24.1+/-2.9 vs. -15.9+/-2.1 mmHg, p