THERMOSPRAY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY OF NUCLEOSIDES AND OF ENZYMATIC HYDROLYSATES OF NUCLEIC-ACIDS
THERMOSPRAY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY OF NUCLEOSIDES AND OF ENZYMATIC HYDROLYSATES OF NUCLEIC-ACIDS
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DOI：
10.1093/nar/13.22.8197
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发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
MCCLOSKEY, JA
中科院分区：
文献类型：
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作者：
EDMONDS, CG;VESTAL, ML;MCCLOSKEY, JA
溶解在水缓冲溶液中的核苷在使用热喷雾界面将溶液直接引入质谱仪时发生电离。形成的主要离子代表质子化的分子、相应的质子化的游离碱和糖。除了对新核苷进行表征的潜在效用外，该技术还可用于通过液相色谱法监测从酶解物中分离的核苷。色谱检测的选择性明显大于单独使用紫外吸光度，因此通常可以独立检测未解析色谱峰的组分。大多数核苷的检测限为信号/噪声bbb10，对于选定的离子监测，每种成分的检测限约为0.1-1 ng，对于全扫描质谱，检测限约为10 - 50 ng。从0.05 a260单位（2.5 ug）兔肝tRNAValand未分离h酶解物中检测修饰核苷的例子。volcaniitRNA。
Nucleosides dissolved in aqueous buffered solutions undergo ionization during direct introduction of the solution into a mass spectrometer using a thermospray interface. The principal ions formed represent the protonated molecule, the corresponding protonated free base, and sugar. In addition to potential utility for characterization of new nucleosides, the technique can be used to monitor nucleosides separated from enzymatic hydrolysates by liquid chromatography. The selectivity of chromatographic detection is significantly greater than with UV absorbance alone so that independent detection of components of unresolved chromatographic peaks is usually possible. Detection limits, with signal/noise >10 for most nucleosides, are approximately 0.1–1 ng per component for selected ion monitoring and 10–50 ng for full-scan mass spectra. Examples are given from the detection of modified nucleosides in enzymatic hydrolysates of 0.05 A260units (2.5 ug) of rabbit liver tRNAValand of unfractionatedH. volcaniitRNA.