PLATELET-ADHESION, SHAPE CHANGE, AND AGGREGATION - RAPID INITIATION AND SIGNAL-TRANSDUCTION EVENTS
PLATELET-ADHESION, SHAPE CHANGE, AND AGGREGATION - RAPID INITIATION AND SIGNAL-TRANSDUCTION EVENTS
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DOI：
10.1139/y94-044
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发表时间：
1994-03-01
影响因子：
2.1
通讯作者：
GEAR, ARL
中科院分区：
文献类型：
--
作者：
GEAR, ARL
血小板对止血至关重要，了解血小板的功能对了解正常和病理情况都很重要。许多方法被用于评估血小板粘附、聚集和分泌，在过去的10年里，人们对这些不同功能阶段发生的生化机制和信号转导事件非常感兴趣。新的信息来自于评估血小板激活后立即发生的变化的技术的发展，这些变化与动脉循环中止血所需的速度一致。使用快速流动和混合技术（如在淬火流、连续流和止流装置中看到的）表明，血小板聚集、形状变化、粘附和分泌在1秒内开始，可能在5秒内几乎完成。诸如蛋白质磷酸化、钙释放和磷脂水解等生化变化在数百毫秒内明显可见。因此，有必要了解信号转导的这些早期事件，并评估可能在糖尿病等疾病中发生的改变。
Blood platelets are essential for hemostasis, and knowledge of their function is important for understanding both normal and pathologic situations. A number of approaches have been used to evaluate platelet adhesion, aggregation, and secretion, and within the last 10 years much interest has been directed to the biochemical mechanisms and signal transduction events occurring during these various phases of function. New information has come from development of technologies to evaluate the changes occurring immediately after platelet activation and consistent with the speed needed for hemostasis in the arterial circulation. Use of rapid flow and mixing technologies as seen in quenched-flow, continuous-flow, and stopped-flow devices has revealed that platelet aggregation, shape change, adhesion, and secretion begin within 1 s and may be nearly complete by 5 s. Biochemical changes such as in protein phosphorylation, calcium release, and phospholipid hydrolysis are clearly evident in hundreds of milliseconds. Therefore, it is necessary to understand these early events in signal transduction and to assess alterations that may occur in diseases such as diabetes.