Associative learning disrupted by impaired G(s) signaling in Drosophila mushroom bodies
Associative learning disrupted by impaired G(s) signaling in Drosophila mushroom bodies
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DOI：
10.1126/science.274.5295.2104
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发表时间：
1996-12-20
期刊：
影响因子：
56.9
通讯作者：
OKane, CJ
中科院分区：
文献类型：
--
作者：
Connolly, JB;Roberts, IJH;OKane, CJ
蘑菇体（MB）或中央复合体（CC）脑结构的破坏会损害果蝇的联想嗅觉学习。腺苷3'，5 '单磷酸信号的扰动也会破坏学习。为了整合这些观察结果，将组成性激活的刺激性异源三聚体三磷酸鸟苷结合蛋白α亚基（G α（s）（星星））的表达靶向这些脑结构。当G α（s）（星星）靶向MB而不是CC结构时，将气味与电休克相关联的能力被消除，而对这些刺激的感觉运动反应保持正常。G α（s）（星星）的表达不影响总MB形态，并且野生型G α（s）表达不影响学习。因此，嗅觉学习依赖于果蝇MB中调节的G（s）信号传导。
Disruptions in mushroom body (MB) or central complex (CC) brain structures impair Drosophila associative olfactory learning. Perturbations in adenosine 3',5' monophosphate signaling also disrupt learning. To integrate these observations, expression of a constitutively activated stimulatory heterotrimeric guanosine triphosphate-binding protein alpha subunit (G alpha(s)(star)) was targeted to these brain structures. The ability to associate odors with electroshock was abolished when G alpha(s)(star) was targeted to MB, but not CC, structures, whereas sensorimotor responses to these stimuli remained normal, Expression of G alpha(s)(star) did not affect gross MB morphology, and wild-type G alpha(s) expression did not affect learning, Thus, olfactory learning depends on regulated G(s) signaling in Drosophila MBs.