Griffonia simplicifolia isolectin B4 identifies a specific subpopulation of angiogenic blood vessels following contusive spinal cord injury in the adult mouse
Griffonia simplicifolia isolectin B4 identifies a specific subpopulation of angiogenic blood vessels following contusive spinal cord injury in the adult mouse
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DOI：
10.1002/cne.21570
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发表时间：
2008-03-01
影响因子：
2.5
通讯作者：
Whittemore, Scott R.
中科院分区：
文献类型：
--
作者：
Benton, Richard L.;Maddie, Melissa A.;Whittemore, Scott R.
创伤性脊髓损伤 (SCI) 后，受损脊髓组织内微脉管系统的破坏和可塑性导致与原发性和继发性损伤的演变相关的病理级联反应。相反，保留血管功能很可能导致组织保留和随后的功能恢复。在细胞水平上识别受损或再生血管的亚类一直很困难。在此，成年小鼠在中度胸部 (T9) 挫伤后 1-28 天接受单次静脉注射 Griffonia simplicifolia isolectin B4 (1134)。 1134 的血管结合在损伤后 7 天时最多观察到，该时间与内在适应性血管生成的多个病理方面相关，1134 血管分布的数量在损伤后 21 天减少。对 1134 结合的定量评估表明，它发生在不断发展的病变中心内，受影响的血管表达暂时特异性功能失调的紧密连接表型，如通过 occludin、claudin-5 和 ZO-1 免疫反应性评估的。综上所述，这些结果表明 SCI 后血管内凝集素递送不仅对于观察新生血管形成的功能状态而且对于明确识别反应性脊髓微血管元件的特定亚群而言是一种有用的方法。 J.Comp。内罗尔。 507:1031-1052, 2008。(c) 2007 Wiley-Liss, Inc.
After traumatic spinal cord injury (SCI), disruption and plasticity of the microvasculature within injured spinal tissue contribute to the pathological cascades associated with the evolution of both primary and secondary injury. Conversely, preserved vascular function most likely results in tissue sparing and subsequent functional recovery. It has been difficult to identify subclasses of damaged or regenerating blood vessels at the cellular level. Here, adult mice received a single intravenous injection of the Griffonia simplicifolia isolectin B4 (1134) at 1-28 days following a moderate thoracic (T9) contusion. Vascular binding of 1134 was maximally observed 7 days following injury, a time associated with multiple pathologic aspects of the intrinsic adaptive angiogenesis, with numbers of 1134 vascular profiles decreasing by 21 days postinjury. Quantitative assessment of 1134 binding shows that it occurs within the evolving lesion epicenter, with affected vessels expressing a temporally specific dysfunctional tight junctional phenotype as assessed by occludin, claudin-5, and ZO-1 immunoreactivities. Taken together, these results demonstrate that intravascular lectin delivery following SCI is a useful approach not only for observing the functional status of neovascular formation but also for definitively identifying specific subpopulations of reactive spinal microvascular elements. J. Comp. Neurol. 507:1031-1052, 2008. (c) 2007 Wiley-Liss, Inc.