Roles of the Middle Domain-Specific WUSCHEL-RELATED HOMEOBOX Genes in Early Development of Leaves in Arabidopsis
Roles of the Middle Domain-Specific WUSCHEL-RELATED HOMEOBOX Genes in Early Development of Leaves in Arabidopsis
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DOI：
10.1105/tpc.111.092858
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发表时间：
2012-02-01
期刊：
影响因子：
11.6
通讯作者：
Okada, Kiyotaka
中科院分区：
文献类型：
--
作者：
Nakata, Miyuki;Matsumoto, Noritaka;Okada, Kiyotaka
在开花植物的叶片发育过程中，近轴（上）和远轴（下）侧特异性基因负责叶片的生长，这主要发生在横向方向，边缘发育以及近轴和远轴组织的分化。然而，对潜在的机制知之甚少。在这里，我们表明，两个WUSCHEL相关的HOMEOBOX（WOX）基因，按花（PRS）/WOX 3和WOX 1，编码同源框转录因子，作用于叶片生长和边缘发展下游的近轴/远轴极性建立。PRS和WOX 1的表达定义了一个迄今未描述的中间域，包括两个中间叶肉层和边缘，作为组织叶片生长的中心。PRS和WOX 1的表达在远轴叶结构域中被远轴特异性转录因子KANADI抑制。此外，PRS和WOX 1与近轴和远轴特异性基因一起协调近轴/远轴模式。我们的数据表明了通过拟南芥叶片中中间结构域特异性WOX基因实现叶片生长和近轴/远轴模式的模型。
During leaf development in flowering plants, adaxial (upper) and abaxial (lower) side-specific genes are responsible for blade outgrowth, which takes places predominantly in the lateral direction, and for margin development as well as differentiation of adaxial and abaxial tissues. However, the underlying mechanisms are poorly understood. Here, we show that two WUSCHEL-RELATED HOMEOBOX (WOX) genes, PRESSED FLOWER (PRS)/WOX3 and WOX1, encoding homeobox transcription factors, act in blade outgrowth and margin development downstream of adaxial/abaxial polarity establishment. The expression of PRS and WOX1 defines a hitherto undescribed middle domain, including two middle mesophyll layers and the margin, as a center that organizes the outgrowth of leaf blades. The expression of PRS and WOX1 is repressed in the abaxial leaf domain by the abaxial-specific transcription factor KANADI. Furthermore, PRS and WOX1 coordinate adaxial/abaxial patterning together with adaxial-and abaxial-specific genes. Our data suggest a model of blade outgrowth and adaxial/abaxial patterning via the middle domain-specific WOX genes in Arabidopsis thaliana leaves.