Folic acid supplementation diminishes diabetes- and glucose-induced dysmorphogenesis in rat embryos in vivo and in vitro
Folic acid supplementation diminishes diabetes- and glucose-induced dysmorphogenesis in rat embryos in vivo and in vitro
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DOI：
10.2337/diabetes.54.2.546
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发表时间：
2005-02-01
期刊：
影响因子：
7.7
通讯作者：
Eriksson, UJ
中科院分区：
文献类型：
--
作者：
Wentzel, P;Gäreskog, M;Eriksson, UJ
母亲服用叶酸可降低后代患神经管缺陷 (NTD) 的风险，但叶酸是否在糖尿病妊娠中发挥类似作用尚不清楚。目的是研究向怀孕母亲皮下注射叶酸或将化合物添加到培养基中是否会分别影响因体内暴露于糖尿病或体外高浓度葡萄糖而引起的大鼠胚胎发育不良。与对照胚胎相比，胚胎在体内暴露于母体糖尿病或在体外暴露于 30 mmol/l 葡萄糖会导致畸形率增加（分别为 71% 和 88% NTD），并降低体节数量和冠臀长度。当我们给糖尿病妊娠大鼠注射叶酸，或在高糖培养基中添加2 mmol/l叶酸时，胚胎参数得到改善（分别为3%和5% NTD）。目前的工作表明，服用叶酸可以减少糖尿病引起的发育不良。这表明补充叶酸可能在预防糖尿病妊娠畸形方面发挥作用。
Maternal administration of folic acid diminishes the risk of neural tube defects (NTDs) in offspring, but whether folic acid exerts a similar effect in diabetic pregnancy is unknown. The aim was to investigate whether maldevelopment in rat embryos caused by exposure to diabetes in vivo or high-glucose concentrations in vitro is affected by subcutaneous administration of folic acid to the pregnant mother or by adding the compound to the culture medium, respectively. Exposure of embryos to maternal diabetes in vivo or 30 mmol/l glucose in vitro yielded an increased malformation rate (71 and 88% NTD, respectively) and lowered somite number and crown-rump length compared with control embryos. When we injected folic acid into the diabetic pregnant rat, or added 2 mmol/l folic acid to the culture medium with high glucose, the embryonic parameters improved (3 and 5% NTD, respectively). The present work shows that administration of folic acid can diminish diabetes-induced maldevelopment. This suggests that folic acid supplementation may have a role in the prevention of malformations in diabetic pregnancy.