Sex-peptide is the molecular basis of the sperm effect in Drosophila melanogaster
Sex-peptide is the molecular basis of the sperm effect in Drosophila melanogaster
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DOI：
10.1073/pnas.1631700100
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发表时间：
2003-08-19
影响因子：
11.1
通讯作者：
Kubli, E
中科院分区：
文献类型：
--
作者：
Liu, HF;Kubli, E
交配使许多雌性昆虫的生殖行为发生两大变化。产卵率增加，接受雄性的准备（接受性）降低。这些交配后的反应在果蝇中持续约1周。交配期间不转移精子但转移精液的雄性动物诱导1天的短期反应。1周的长期反应需要精子的存在（精子效应）。因此，精子对于交配后反应的长期持续性至关重要。三种精液肽引起交配后反应：排卵素，性肽（SP），和DUP 998。利用同源重组靶向突变技术，我们产生了具有突变SP基因的雄性。在这里，我们报告说，男性缺乏功能SP引起只有一个弱的短期反应。然而，这些雄性确实会转移精子。因此，（1）SP是引起短期和长期交配后反应的主要媒介，（2）精子只是SP的载体。第二个结论得到了SP与精子结合的发现的支持。编码36-aa的SP基因是第一个用同源重组方法敲除的果蝇小基因。
Mating elicits two major changes in the reproductive behavior of many insect females. The egg-laying rate increases and the readiness to accept males (receptivity) is reduced. These postmating responses last approximate to1 week in Drosophila melanogaster. Males that do not transfer sperm but transfer seminal fluid during mating induce a short-term response of 1 day. The long-term response of 1 week requires the presence of sperm (sperm effect). Hence, sperm is essential for the long-term persistence of the postmating responses. Three seminal fluid peptides elicit postmating responses: ovulin, sex-peptide (SP), and DUP998. Using the technique of targeted mutagenesis by homologous recombination, we have produced males with mutant SP genes. Here, we report that males lacking functional SP elicit only a weak short-term response. However, these males do transfer sperm. Thus, (i) SP is the major agent eliciting the short-term and the long-term postmating responses and (it) sperm is merely the carrier for SP. The second conclusion is supported by the finding that SP binds to sperm. The 36-aa-encoding SP gene is the first small Drosophila gene knocked out with the method of homologous recombination.