Cloning and characterization of a novel human cDNA encoding a J-domain protein (DNAJA5) from the fetal brain.
Cloning and characterization of a novel human cDNA encoding a J-domain protein (DNAJA5) from the fetal brain.
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编码来自胎儿大脑的 J 结构域蛋白 (DNAJA5) 的新型人类 cDNA 的克隆和表征。
DOI：
10.3892/ijmm.13.5.735
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发表时间：
2004
影响因子：
5.4
通讯作者：
Y. Mao
中科院分区：
文献类型：
--
作者：
Juxiang Chen;Gang Yin;Yi;Mei;Haipeng Cheng;X. Ni;G. Hu;C. Luo;K. Ying;Yi Xie;Y. Mao
J结构域蛋白参与蛋白质的转运和分选、蛋白质转运到内质网并与热休克蛋白70相互作用、细胞周期调控和胞吐作用。在对人胎脑cDNA文库进行大规模测序分析的过程中，我们分离到一个cDNA克隆，该克隆编码一个531个氨基酸的蛋白质，具有DnaJ结构域和C2 H2型锌指结构域，命名为DNAJA 5（human DnaJ homology subfamily A member 5）。DNAJA 5基因与秀丽隐杆线虫DNJ-17同源性为33.2%，相似性为45.1%。将DNAJA 5基因定位于人染色体5 p12 - 5 p13。DNAJA 5基因包含至少12个外显子，跨越超过25.6 kb的基因组序列。逆转录-聚合酶链反应分析表明，该基因在人脑、胎盘、肾脏和胰腺中均有表达。DNAJA 5可能在与Hsp 70的协同作用中发挥作用。
J-domain proteins are involved in protein importing and sorting, protein translocation into the endoplasmic reticulum and interaction with hsp70, cell cycle regulation and exocytosis. During the large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a cDNA clone encoding a putative 531 amino acid protein with a DnaJ domain and C2H2-type zinc finger motif, which was named as DNAJA5 (human DnaJ homology subfamily A member 5). The putative protein of DNAJA5 gene was homologous to Caenorhabditis elegans DNJ-17 (identity 33.2% similarity 45.1%). By mapping, we located DNAJA5 gene to human chromosome 5p12-5p13. The DNAJA5 gene contained at least 12 exons that spanned more than 25.6 kb of the genomic sequence. The reverse transcription-polymerase chain reaction analysis indicated that it was expressed in the human brain, placenta, kidney and pancreas. DNAJA5 might play a role in co-operating with Hsp70.