Aptasensor for ATP based on analyte-induced dissociation of ferrocene-aptamer conjugates from manganese dioxide nanosheets on a screen-printed carbon electrode
Aptasensor for ATP based on analyte-induced dissociation of ferrocene-aptamer conjugates from manganese dioxide nanosheets on a screen-printed carbon electrode
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DOI：
10.1007/s00604-016-1916-2
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发表时间：
2016-07
影响因子：
5.7
通讯作者：
D. Tang;Li Hou
中科院分区：
文献类型：
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作者：
D. Tang;Li Hou
作者报道了一种新的电化学适配策略，用于测定皮摩尔水平的腺苷-5 '-三磷酸（ATP）。首先，基于高锰酸钾与阳离子洗涤剂十六烷基三甲基溴化铵的反应，采用热注入法合成了平均粒径约为70 nm的二氧化锰纳米片。然后将所得的MnO 2纳米片固定到预处理的丝网印刷碳电极上，该碳电极通过核碱基和纳米片的基面之间的货车德瓦尔斯力容易地结合二茂铁标记的ATP适体。固定化的二茂铁-适体缀合物激活与电极的电接触，并在扫描的电势中产生强信号（0.0至1.0V vs. Ag/AgCl）。加入ATP后，它将与适体反应并导致二茂铁适体从纳米片解离，这导致电信号减少。在最佳条件下，该平台显示出低至0.32 nM的ATP检测限。在10 nM浓度水平下，重复性和中间精密度低于10.7%。该方法用于分析加标ATP的空白胎牛血清，回收率（3个浓度水平）范围为91.3%至118%。该检测方案快速、简单、成本低，不需要大量的样品制备或多次洗涤步骤。图形摘要基于二茂铁标记的适配体的靶诱导解离，MnO 2纳米片被用作电化学检测ATP的传感平台。
The authors report on a new electrochemical aptasensing strategy for the determination of adenosine - 5’-triphosphate (ATP) at picomolar levels. First, manganese dioxide (MnO2) nanosheets with an average size of ~70 nm were synthesized via a hot-injection method on the basis of reaction between potassium permanganate and the cationic detergent cetyltrimethylammonium bromide. The resulting MnO2nanosheets were then immobilized onto a pretreated screen-printed carbon electrode which readily binds the ferrocene-labeled ATP aptamer through the van der Waals force between the nucleobases and the basal plane of the nanoflakes. The immobilized ferrocene-aptamer conjugates activates the electrical contact with the electrode and produces a strong signal in the potentials scanned (0.0 to 1.0 V vs. Ag/AgCl). Upon addition of ATP, it will react with the aptamer and cause the dissociation of the ferrocene-aptamer from the nanosheets, this resulting in a decrease in the electrical signal. Under optimal conditions, this platform exhibits a detection limit as low as 0.32 nM of ATP. The repeatability and intermediate precision is below 10.7 % at a 10 nM concentration level. The method was applied to analyze blank fetal calf serum spiked with ATP, and the recoveries (at 3 concentration levels) ranged between 91.3 and 118 %. This detection scheme is rapid, simple, cost-effective, and does not require extensive sample preparation or multiple washing steps.Graphic abstractMnO2nanosheets are used as the sensing platform for electrochemical detection of ATP based on target-induced dissociation of ferrocene-labeled aptamer.