Sequence dependence of conformations of self-complementary duplex tetradeoxynucleotides containing cytosine and guanine.
Sequence dependence of conformations of self-complementary duplex tetradeoxynucleotides containing cytosine and guanine.
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含有胞嘧啶和鸟嘌呤的自互补双链体十四氧核苷酸的构象的序列依赖性。
DOI：
10.1021/bi00309a014
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Peticolas,WL
中科院分区：
文献类型：
--
作者：
Thomas,GA;Peticolas,WL
Gerald A.Thomas和Warner L.Peticolas**摘要：四个只含有胞嘧啶和鸟氨酸的自互补四脱氧核苷酸分别是5‘-d-(CpGpCpG)-3’，5，-d(CpCpGpG)-3‘，5’-d(GpCpGpC)-3‘和5’-d(GpGpCpC)-3‘。这些四核苷酸的水溶液(约0.05M单体)在pH 7和2C下的拉曼光谱清楚地表明，当氯化钠浓度为0.5M时，这些自互补的四核苷酸形成典型的B型双链双链结构。如果温度升高到50℃，拉曼光谱显示，在每种情况下，双螺旋B型都以不合作的方式熔化成无序的单链形式。另一方面，如果盐浓度增加到饱和，这四个四核苷酸中只有一个的拉曼光谱
Gerald A. Thomas and Warner L. Peticolas** abstract: The four self-complementary tetradeoxynucleotides which contain only cytosineand guanine are 5'-d-(CpGpCpG)-3', 5,-d (CpCpGpG)-3', 5'-d (GpCpGpC)-3', and 5'-d (GpGpCpC)-3'. The Raman spectra of aqueous solutions (about 0.05 M inmonomer) of these tetranucleotides at pH 7 and 2 C show clearly that these self-complementary tet-ranucleotides form double-stranded duplex structures of the canonical B type when the NaCl concentration is 0.5 M NaCl. If the temperature is raised to 50 C, the Ramanspectra show that in each case the double-helical B form melts in a non-cooperative way to a disordered single-chain form. On the other hand, if the salt concentration is raised to saturation, the Raman spectrum of only one of these four tetranucleotide