Isolation of microtubule protein from mammalian brain frozen for extended periods of time.
Isolation of microtubule protein from mammalian brain frozen for extended periods of time.
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DOI：
10.1016/1046-5928(91)90099-5
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发表时间：
1991-10-01
影响因子：
1.6
通讯作者：
Wolff, J
中科院分区：
文献类型：
--
作者：
Sackett, D L;Knipling, L;Wolff, J
微管蛋白（MTP）可以通过温度依赖性聚合和解聚的循环从冷冻脑中以良好的产率分离。如果大脑快速冷冻并在-70 ℃下储存，MTP的产量至少稳定2个月，近一年后产量仅略有下降。牛脑和大鼠脑都可以用这种方式储存，前提是采取适当的预防措施以确保牛脑的快速冷冻。该程序允许在一段时间内积累大脑，以便在以后方便的日期进行MTP分离。
Microtubule protein (MTP) may be isolated in good yield from frozen brains by cycles of temperature-dependent polymerization and depolymerization. If the brains are frozen quickly and stored at -70 degrees C, the yield of MTP is stable for a period of at least 2 months and the yield is only slightly decreased after nearly a year. Cow as well as rat brains may be stored in this manner, provided appropriate precautions are taken to ensure rapid freezing of the cow brain. This procedure allows brains to be accumulated over a period of time for MTP isolation at a convenient later date.