Organ-Specific Analysis of Morus alba Using a Gel-Free/Label-Free Proteomic Technique
Organ-Specific Analysis of Morus alba Using a Gel-Free/Label-Free Proteomic Technique
复制标题
使用无凝胶/无标记蛋白质组技术对桑树进行器官特异性分析
DOI：
10.3390/ijms20020365
复制
发表时间：
2019-01-02
影响因子：
5.6
通讯作者：
Tian, Jingkui
中科院分区：
文献类型：
--
作者：
Zhu, Wei;Zhong, Zhuoheng;Tian, Jingkui
桑树是一种重要的药用植物，用于治疗人类疾病。桑叶、桑枝和桑根分别可用作降糖尿病、抗氧化和抗炎药物。为了探索桑树不同部位不同药理作用的分子机制，进行了器官特异性蛋白质组学研究。用无凝胶/无标记蛋白质组学技术测定了桑叶、枝条和根的蛋白质谱。在桑叶、桑枝和桑根中，分别鉴定出492、414和355个蛋白质，其中包括84个共同蛋白质。在叶片中，其主要功能与蛋白质降解、光合作用和氧化还原抗坏血酸/谷胱甘肽代谢有关。在枝条中，主要功能与蛋白质合成/降解、胁迫和氧化还原抗坏血酸/谷胱甘肽代谢有关。在根中，其主要功能与蛋白质合成/降解、胁迫和细胞壁有关。此外，还分析了特定器官的代谢物和抗氧化活性。这些结果表明，与桑枝和桑叶相比，桑根中黄酮类化合物的积累较高。相应地，在类黄酮途径中积累了查尔酮-黄烷酮异构酶和类黄酮3，5-羟基酶两种根特异蛋白质。与此一致，根中的总黄酮含量高于枝叶中的含量。这些结果表明，桑根中的黄酮类化合物可能与其生物活性有关，桑根是桑黄酮类化合物生物合成的主要部分。
Morus alba is an important medicinal plant that is used to treat human diseases. The leaf, branch, and root of Morus can be applied as antidiabetic, antioxidant, and anti-inflammatory medicines, respectively. To explore the molecular mechanisms underlying the various pharmacological functions within different parts of Morus, organ-specific proteomics were performed. Protein profiles of the Morus leaf, branch, and root were determined using a gel-free/label-free proteomic technique. In the Morus leaf, branch, and root, a total of 492, 414, and 355 proteins were identified, respectively, including 84 common proteins. In leaf, the main function was related to protein degradation, photosynthesis, and redox ascorbate/glutathione metabolism. In branch, the main function was related to protein synthesis/degradation, stress, and redox ascorbate/glutathione metabolism. In root, the main function was related to protein synthesis/degradation, stress, and cell wall. Additionally, organ-specific metabolites and antioxidant activities were analyzed. These results revealed that flavonoids were highly accumulated in Morus root compared with the branch and leaf. Accordingly, two root-specific proteins named chalcone flavanone isomerase and flavonoid 3,5-hydroxylase were accumulated in the flavonoid pathway. Consistent with this finding, the content of the total flavonoids was higher in root compared to those detected in branch and leaf. These results suggest that the flavonoids in Morus root might be responsible for its biological activity and the root is the main part for flavonoid biosynthesis in Morus.