Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli
Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli
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DOI：
10.1073/pnas.252529799
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发表时间：
2002-12-24
影响因子：
11.1
通讯作者：
Blattner, FR
中科院分区：
文献类型：
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作者：
Welch, RA;Burland, V;Blattner, FR
我们给出了泌尿系致病大肠杆菌CFT073株的全基因组序列。令人惊讶的是，CFT073、肠出血性大肠杆菌EDL933和实验室菌株MG1655的三向基因组比较表明，它们结合的(非冗余)蛋白质组中实际上只有39.2%是所有三个菌株共同的。病原体基因组彼此不同，就像每一种病原体来自良性菌株一样。O157：H7和CFT073之间致病潜力的差异反映在缺乏III型分泌系统的基因或在某些类别的致泻性大肠杆菌中发现的噬菌体和质粒编码的毒素。CFT073基因组特别丰富，编码潜在的菌毛粘附素、自体转运蛋白、铁封存系统和相切换重组酶。CFT073的大毒力岛与另外两株研究得很好的泌尿系致病大肠杆菌J96和536之间存在着显著的差异。比较表明，肠外致病性大肠埃希菌独立于多个克隆谱系。不同的大肠杆菌致病类型保持了共同的、垂直进化的基因的显著一致性，而许多干扰这一共同骨架的岛屿是通过每个菌株中不同的水平转移事件获得的。
We present the complete genome sequence of uropathogenic Escherichia coli, strain CFT073. A three-way genome comparison of the CFT073, enterohemorrhagic E. coli EDL933, and laboratory strain MG1655 reveals that, amazingly, only 39.2% of their combined (nonredundant) set of proteins actually are common to all three strains. The pathogen genomes are as different from each other as each pathogen is from the benign strain. The difference in disease potential between O157:H7 and CFT073 is reflected in the absence of genes for type III secretion system or phage- and plasmid-encoded toxins found in some classes of diarrheagenic E. coli. The CFT073 genome is particularly rich in genes that encode potential fimbrial adhesins, autotrans porters, iron-sequestration systems, and phase-switch recombinases. Striking differences exist between the large pathogenicity islands of CFT073 and two other well-studied uropathogenic E. coli strains, J96 and 536. Comparisons indicate that extraintestinal pathogenic E. coli arose independently from multiple clonal lineages. The different E. coli pathotypes have maintained a remarkable synteny of common, vertically evolved genes, whereas many islands interrupting this common backbone have been acquired by different horizontal transfer events in each strain.