PERTURBATION OF THE UBIQUITIN SYSTEM CAUSES LEAF CURLING, VASCULAR TISSUE ALTERATIONS AND NECROTIC LESIONS IN A HIGHER-PLANT
PERTURBATION OF THE UBIQUITIN SYSTEM CAUSES LEAF CURLING, VASCULAR TISSUE ALTERATIONS AND NECROTIC LESIONS IN A HIGHER-PLANT
复制标题
DOI：
10.1002/j.1460-2075.1990.tb07906.x
复制
发表时间：
1990-12-01
期刊：
影响因子：
11.4
通讯作者：
SCHELL, J
中科院分区：
文献类型：
--
作者：
BACHMAIR, A;BECKER, F;SCHELL, J
Lys48变为Arg的泛素变体在体外作为泛素依赖性蛋白质降解的抑制剂。 为了评估这种蛋白水解途径在植物生命周期中的作用，我们在烟草中表达了泛素变体。 变异的单或多泛素的表达导致血管组织的显著异常。 此外，变体多聚泛素的过表达诱导叶片上的离散病变。 这表明泛素系统的扰动可以诱导植物的程序性坏死反应。
A ubiquitin variant with Lys48 changed to Arg acts in vitro as an inhibitor of ubiquitin dependent protein degradation. To assess the role of this proteolytic pathway in the life cycle of plants, we expressed the ubiquitin variant in Nicotianna tabacum. Expression of variant mono- or polyubiquitin leads to marked abnormalities in vascular tissue. In addition, overexpression of variant polyubiquitin induces discrete lesions on leaves. This indicates that perturbations of the ubiquitin system can induce a programmed necrotic response in plants.