Single neuron analysis by capillary electrophoresis with fluorescence spectroscopy
Single neuron analysis by capillary electrophoresis with fluorescence spectroscopy
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DOI：
10.1016/s0896-6273(00)80446-8
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发表时间：
1998-02-01
期刊：
影响因子：
16.2
通讯作者：
Sweedler, JV
中科院分区：
文献类型：
--
作者：
Fuller, RR;Moroz, LL;Sweedler, JV
描述了一种同时识别和定量单个神经元中30多种化合物的技术。该方法采用纳升体积采样，毛细管电泳分离，波长分辨天然荧光检测。检测限（LOD）范围从低阿托摩尔到飞摩尔，5-羟色胺（或5-羟色胺[5-HT]）LOD与20阿托摩尔相似。虽然细胞样品基质是化学复杂的，电泳迁移时间和荧光光谱信息的组合允许芳香族单胺，芳香族氨基酸和含有它们的肽，黄素，腺苷和鸟苷核苷酸类似物，和其他荧光化合物的阳性鉴定。个别确定的神经元，从Aaplasia californica和Pleurobranchaea californica被用来证明这种技术的适用性和优点的数字。
A technique to identify and quantitate simultaneously more than 30 compounds in individual neurons is described. The method uses nanoliter volume sampling, capillary electrophoresis separation, and wavelength-resolved native fluorescence detection. Limits of detection (LODs) range from the low attomole to the femtomole range, with 5-hydroxytryptamine (or serotonin [5-HT]) LODs being similar to 20 attomoles. Although the cellular sample matrix is chemically complex, the combination of electrophoretic migration time and fluorescence spectral information allows positive identification of aromatic monoamines, aromatic amino acids and peptides containing them, flavins, adenosine- and guanosine-nucleotide analogs, and other fluorescent compounds. Individual identified neurons from Aplysia californica and Pleurobranchaea californica are used to demonstrate the applicability and figures of merit of this technique.