Functional magnetic resonance microscopy at single-cell resolution in Aplysia californica
Functional magnetic resonance microscopy at single-cell resolution in Aplysia californica
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DOI：
10.1073/pnas.1403739111
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发表时间：
2014-06-10
影响因子：
11.1
通讯作者：
Ciobanu, Luisa
中科院分区：
文献类型：
--
作者：
Radecki, Guillaume;Nargeot, Romuald;Ciobanu, Luisa
在这项工作中，我们展示了以单细胞分辨率进行功能性 MRI 研究的可行性。在超高磁场下，锰增强磁共振显微镜可以在低、无毒的 Mn2+ 浓度下识别海兔颊侧网络中的大多数运动神经元。我们确定，Mn2+在注射到活体海兔体内后会在细胞内积累，并且当动物受到感官刺激时，其浓度会增加。我们还表明，我们可以区分不同类型的刺激引起的神经元活动。这种方法开辟了一条新途径，以单细胞分辨率探讨涉及目标导向行为的神经元网络的功能组织和可塑性。
In this work, we show the feasibility of performing functional MRI studies with single-cell resolution. At ultrahigh magnetic field, manganese-enhanced magnetic resonance microscopy allows the identification of most motor neurons in the buccal network of Aplysia at low, nontoxic Mn2+ concentrations. We establish that Mn2+ accumulates intracellularly on injection into the living Aplysia and that its concentration increases when the animals are presented with a sensory stimulus. We also show that we can distinguish between neuronal activities elicited by different types of stimuli. This method opens up a new avenue into probing the functional organization and plasticity of neuronal networks involved in goal-directed behaviors with single-cell resolution.