TEMPERATURE-MEDIATED PROCESSES IN TELEOST IMMUNITY - DIFFERENTIAL-EFFECTS OF INVITRO AND INVIVO TEMPERATURES ON MITOGENIC RESPONSES OF CHANNEL CATFISH LYMPHOCYTES
TEMPERATURE-MEDIATED PROCESSES IN TELEOST IMMUNITY - DIFFERENTIAL-EFFECTS OF INVITRO AND INVIVO TEMPERATURES ON MITOGENIC RESPONSES OF CHANNEL CATFISH LYMPHOCYTES
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DOI：
10.1016/0145-305x(84)90038-7
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发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
CUCHENS, MA
中科院分区：
文献类型：
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作者：
CLEM, LW;FAULMANN, E;CUCHENS, MA
体内外温度对斑点叉尾鱼外周血白细胞(PBL)对刀豆蛋白A(ConA)和脂多糖(LPS)的促有丝分裂反应有不同的影响。对内毒素的反应大小与体外培养温度和体内驯化温度均相对独立。对ConA的反应在较低的体外温度下受到抑制，尽管这种抑制可以通过较低的体内驯化温度来降低。体外温移实验表明，在较高的体外温度下，首先用ConA刺激斑点叉尾轮鱼PBL可以在较低的体外温度下对ConA产生反应。相反，斑点叉尾鱼PBL在较高的体外温度下，在较低的体外温度下接受ConA的初始刺激后，不会产生反应。后一种反应失败不能归因于ConA在较低温度下诱导抑制细胞(或因子)。这些研究，再加上斑点叉尾鱼PBL亚群的其他现有数据，被解释为支持这样的假设，即鱼类的低温免疫抑制可能是由于对T细胞而不是B细胞的优先抑制作用。
The in vitro [PBL] mitogenic responses of channel catfish peripheral blood leukocytes [PBL] to ConA [concanavalin A] and LPS [lipopolysaccharide] were differentially affected by both in vitro and in vivo temperatures. The magnitude of the response to LPS was relatively independent of both in vitro culture temperature and in vivo acclimation temperature. The magnitude of the response to ConA was suppressed at lower in vitro temperatures although this suppression could be reduced by lower in vivo acclimation temperatures. In vitro temperature-shift experiments indicated that channel catfish PBL could respond to ConA at a lower in vitro temperature if first stimulated with ConA at a higher in vitro temperature. The converse was not true in that channel catfish PBL did not respond at a higher in vitro temperature after an initial stimulation with ConA at a lower in vitro temperature. This latter failure to respond could not be attributed to the induction of a suppressor cell (or factor) by exposure to ConA at a lower temperature. The studies, when coupled with other available data on channel catfish PBL subpopulations, are interpreted as supporting the hypothesis that low temperature immunosuppression in fish may result from preferential inhibitory effects on T cells rather than B cells.