Characterization of a novel Tombusviridae species isolated from Paris polyphylla var. yunnanensis
Characterization of a novel Tombusviridae species isolated from Paris polyphylla var. yunnanensis
复制标题
从巴黎一枝花中分离出的一种新的 Tombusviridae 物种的特征
DOI：
10.1007/s00705-021-05191-y
复制
发表时间：
2021-08-13
影响因子：
2.7
通讯作者：
Zhao, Mingfu
中科院分区：
文献类型：
--
作者：
Chen, Lu;Anane, Rex Frimpong;Zhao, Mingfu
从多叶重楼（巴黎polyphylla var.）测定并分析了其全基因组序列。ParV 2是一种正义单链RNA（+ssRNA）病毒，基因组大小为4，118个核苷酸。ParV 2基因组包含6个推定的开放阅读框（ORF），其编码预测分子量为40.14、100.26、7.31、7.85、26.09和8.77 kDa的蛋白质。ParV 2的第一个ORF（ORF 1）编码一个40.14 kDa的蛋白质（P40，nt：20- 1，096），第二个ORF（ORF 2，888 aa）含有GDD基序，编码高度保守的Tombusviridae病毒依赖RNA的RNA聚合酶蛋白（RdRP，nt：20- 2，683，P100，100.26 kDa）。多重序列比对分析表明，ParV 2的全基因组序列与Tombusviridae病毒的核苷酸序列同源性为31.7-55.5%。姜褪绿斑点相关的番茄丛矮病毒（GCFaV-1，登录号QKE 30557）与ParV 2具有最高的序列同一性（55.5%）。GCFaV-1还与ParV 2共享59.2%的RdRP和34.9%的CP氨基酸序列同一性。RdRP的序列比较和系统发育分析表明，ParV 2是Tombusviridae家族的新成员，其最近的已知亲属是GCFaV-1。
A novel virus, Paris virus 2 (ParV2), was isolated from diseased Paris polyphylla var. yunnanensis, and its complete genome sequence was determined and analyzed. ParV2 is a positive-sense single-stranded RNA (+ssRNA) virus with a genome size of 4,118 nucleotides. The ParV2 genome contains six putative open reading frames (ORFs) that encode proteins with predicted molecular weights of 40.14, 100.26, 7.31, 7.85, 26.09, and 8.77 kDa. The first ORF (ORF1) of ParV2 encodes a putative protein of 40.14 kDa (P40, nt: 20-1,096), whiles the second ORF (ORF2, 888 aa) containing the GDD motif encodes the highly conserved RNA-dependent RNA polymerase protein (RdRP, nt:20-2,683, P100, 100.26 kDa) of viruses in the family Tombusviridae. Multiple sequence alignments analysis showed that the complete genome sequence of ParV2 shares 31.7-55.5% nucleotide sequence identities with viruses in the family Tombusviridae. Ginger chlorotic fleck-associated tombusvirus (GCFaV-1, Accession No. QKE30557) had the highest sequence identity (55.5%) with ParV2. GCFaV-1 also shares 59.2% RdRP and 34.9% CP amino acid sequence identities with ParV2. Sequence comparisons and phylogenetic analysis of RdRP suggested that ParV2 is a novel member of the family Tombusviridae, and its closest known relative is GCFaV-1.