Effect of fasting on the peripheral circadian gene expression in rats
Effect of fasting on the peripheral circadian gene expression in rats
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DOI：
10.1080/09291010902863289
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发表时间：
2010-02
影响因子：
1.1
通讯作者：
Yue Dong;Tao Wu;Y. Ni;H. Kato;Z. Fu
中科院分区：
文献类型：
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作者：
Yue Dong;Tao Wu;Y. Ni;H. Kato;Z. Fu
目前的研究发现，禁食并没有显着改变生物钟基因（Bmal 1，Cry 1，Per 1，Per 2，Per 3，Dec 1，Dec 2和Rev-erb a）和时钟控制基因DBP的昼夜节律相位。然而，这些昼夜节律基因的mRNA水平以基因和组织特异性方式改变。Bmal 1和Cry 1对禁食信号不敏感，因为它们的mRNA水平在禁食后几乎保持不变。相反，Per 2，Dec 1和Dec 2的mRNA水平不仅明显改变，而且在肝脏和心脏中也发生了稳定的变化，表明这三个时钟基因对禁食刺激敏感。而Per 1、Per 3和Rev-erbα在肝脏和心脏中受禁食刺激的调节是不同的，甚至是相反的，提示这三个时钟基因可能在肝脏和心脏的昼夜节律系统中发挥不同的作用。因此，食物线索的存在对于维持外周生物钟的正常昼夜节律水平可能是重要的。
The present study found that fasting did not significantly shift the circadian phases of the clock genes (Bmal1, Cry1, Per1, Per2, Per3, Dec1, Dec2, and Rev-erb a) and clock-controlled gene Dbp. However, the mRNA levels of these circadian genes were altered in both a gene- and tissue-specific manner. Bmal1 and Cry1 were insensitive to the fasting signal, because their mRNA levels almost remained unchanged after fasting. On the contrary, the mRNA levels of Per2, Dec1 and Dec2 were not only markedly altered but also altered steadily in the liver and heart, indicating that these three clock genes are sensitive to the stimuli of fasting. As for Per1, Per3 and Rev-erbα, they were differently or even contrarily regulated in the liver and heart by the stimuli of fasting, suggesting that these three clock genes may play different roles in the circadian systems of the liver and heart. Thus, the existence of food cues may be important for maintaining a regular circadian level in the peripheral clocks.