Biosynthesis of spathulenol and camphor stand as a competitive route to artemisinin production as revealed by a new chemometric convergence approach based on nine locations' field-grown Artemesia annua L.
Biosynthesis of spathulenol and camphor stand as a competitive route to artemisinin production as revealed by a new chemometric convergence approach based on nine locations' field-grown Artemesia annua L.
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基于九个地点的田间种植的青蒿的新化学计量学融合方法揭示了 Spathulenol 和樟脑的生物合成是青蒿素生产的竞争途径。
DOI：
10.1016/j.indcrop.2019.05.056
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发表时间：
2019
影响因子：
5.9
通讯作者：
Chemat S
中科院分区：
文献类型：
--
作者：
Chemat S
由于异戊烯基二磷酸（IPP）及其异构体二甲基烯丙基二磷酸（DMAPP）是两种精油组分和抗疟剂青蒿素及其衍生物的通用前体，本文旨在通过对青蒿的筛选，找出它们在浓度上的斑点差异。主成分分析（PCA）结果表明，青蒿素与β-蒎烯、1，8-桉叶油素、水合桧烯、β-桉叶油素和1-辛烯-3-醇正相关，而与青蒿酸和β-香叶烯氧化物负相关。层次聚类分析（Hierarchical clustering analysis，HCA）将青蒿素浓度作为主要驱动因素的位置分为两类，同时提出了一种改进的基于特异质相似性的收敛方法，该方法能够捕获个体间的异质性，能够将化合物分为4个不同的聚类。青蒿素可与对伞花烃、乙酸顺式香芹酯、4-萜品烯-1-醇、β-香芹烯、β-法呢烯、β-芹烯、α-芹烯、β-氧化香芹烯和α-木香醇交联。有趣的是，看看樟脑和spathulenol如何表现为一个独特的簇群，这表明这两种化合物的生物合成遵循不同但竞争的途径;因此限制它们的生产可能是控制和提高青蒿素生产的关键。
Since isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP) are the universal precursors of both essential oil components, and the antimalarial agent artemisinin and its derivatives inArtemesia annuaL., this paper aims to correlate the spotted differences in their concentrations by screeningArtemesia annuaL. field-grown in nine locations around the world that may reveal the role of any these compounds as precursors or competitors in the biosynthetic pathway of the sesquiterpene lactone : artemisinin.Principal component analysis (PCA) revealed that artemisinin is positively correlated to β-pinene, 1,8-cineole, sabinene hydrate, borneol and 1-octen-3-ol; but negatively to artemisinic acid and β-caryophyllene oxide. Hierarchical cluster analysis (HCA) classified locations into two distinct groups in which artemisinin concentration stood as the main driving factor to build similarities between the locations.In parallel, an improved convergence approach based on idiosyncratic similarities able to capture heterogeneity across individuals is proposed, which was able to classify compounds into four distinct clusters. Artemisinin appeared to be cross-linked to p-cymene, cis-carvyle acetate, 4-terpinene-1-ol, β-caryophyllene, β-farnesene, β-selinene, α-selinene, β-caryophyllene oxide and α-costol. It is interesting to see how camphor and spathulenol behaved as a distinct cluster group, which suggests that biosynthesis of these two compounds follows a different but a competitive pathway ; thus limiting their production could be a key to control and enhance the production of artemisinin.