PRODUCTION OF TUMOR NECROSIS FACTOR AND OTHER CYTOKINES BY ASTROCYTES STIMULATED WITH LIPOPOLYSACCHARIDE OR A NEUROTROPIC VIRUS
PRODUCTION OF TUMOR NECROSIS FACTOR AND OTHER CYTOKINES BY ASTROCYTES STIMULATED WITH LIPOPOLYSACCHARIDE OR A NEUROTROPIC VIRUS
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DOI：
10.1073/pnas.86.16.6348
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发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
SHIN, ML
中科院分区：
文献类型：
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作者：
LIEBERMAN, AP;PITHA, PM;SHIN, ML
大鼠星形胶质细胞是中枢神经系统（CNS）中具有免疫活性的胶质细胞，活化后可释放多种细胞因子。脂多糖刺激的星形胶质细胞产生肿瘤坏死因子（TNF），如使用小鼠TNF探针的北方印迹分析和功能测定所示。大鼠星形胶质细胞源性TNF的生物学活性被兔抗重组鼠TNF的抗血清中和。脂多糖刺激星形胶质细胞也激活了白细胞介素1和白细胞介素6基因。我们还研究了是否嗜神经副粘病毒，纽卡斯尔病病毒，触发星形胶质细胞的细胞因子的生产。该病毒诱导星形胶质细胞产生TNF、光毒素、白细胞介素6和α-TNF。和β-干扰素因此，内毒素和病毒的刺激激活了不同但重叠的细胞因子基因组。我们建议，星形胶质细胞和它们产生的细胞因子可能在免疫和/或病毒介导的CNS疾病的发病机制中发挥重要作用，在CNS细胞间的通信，并在神经和免疫系统之间的相互作用。
Rat astrocytes, immunologically competent glial cells of the central nervous system (CNS), released a variety of cytokines after activation. Lipopolysaccharide-stimulated astrocytes produced tumor necrosis factor (TNF) as demonstrated by Northern blot analysis using a mouse TNF probe and by functional assay. Biological activity of rat astrocyte-derived TNF was neutralized by rabbit antiserum against recombinant murine TNF. Stimulation of astrocytes by lipopolysaccharide also activated the interleukin 1 and interleukin 6 genes. We have also investigated whether a neurotropic paramyxovirus, Newcastle disease virus, triggers cytokine production by astrocytes. This virus induced astrocytes to produce TNF, lymphotoxin, interleukin 6, and .alpha.- and .beta.-interferons. Thus, stimulation by endotoxin and virus activated distinct, yet overlapping, sets of cytokine genes. We propose that astrocytes and the cytokines they produce may play a significant role in the pathogenesis of immunologically and/or virally mediated CNS disease, in CNS intercellular communication, and in the interactions between the nervous and immune systems.