A novel, cell-permeable, fluorescent probe for ratiometric imaging of zinc ion
A novel, cell-permeable, fluorescent probe for ratiometric imaging of zinc ion
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DOI：
10.1021/ja026442n
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发表时间：
2002-09-11
影响因子：
15
通讯作者：
Nagano, T
中科院分区：
文献类型：
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作者：
Maruyama, S;Kikuchi, K;Nagano, T
Zn 2+在各种生物系统中起着重要作用，因此，开发能够可视化可螯合Zn 2+的工具最近引起了人们的广泛关注。我们在这里报告新合成的荧光传感器的Zn 2+，ZnAF-Rs，其激发最大值移动的Zn 2+在生理条件下。因此，这些传感器能够实现比率成像，这是一种通过最小化外来因素对探针荧光的影响来减少伪影的技术。比率测量可以提供精确的数据，并且一些探针允许定量检测。ZnAF-Rs是第一个比率荧光传感器的Zn 2+，使定量分析在生理条件下。ZnAF-Rs还具有合适的Kd值，对其它生物相关阳离子，特别是Ca ~（2+）具有高选择性。用这些探针成功地监测了培养细胞内Zn ~（2+）浓度的变化。我们相信，这些探针将是非常有用的研究锌的生物功能。
Zn2+plays important roles in various biological systems; as a result, the development of tools that can visualize chelatable Zn2+has attracted much attention recently. We report here newly synthesized fluorescent sensors for Zn2+, ZnAF-Rs, whose excitation maximum is shifted by Zn2+under physiological conditions. Thus, these sensors enable ratiometric imaging, which is a technique to reduce artifacts by minimizing the influence of extraneous factors on the fluorescence of a probe. Ratiometric measurement can provide precise data, and some probes allow quantitative detection. ZnAF-Rs are the first ratiometric fluorescent sensors for Zn2+that enable quantitative analysis under physiological conditions. ZnAF-Rs also possess suitableKdfor applications, and high selectivity against other biologically relevant cations, especially Ca2+. Using these probes, changes of intracellular Zn2+concentration in cultured cells were monitored successfully. We believe that these probes will be extremely useful in studies on the biological functions of Zn2+.