A system for precise temperature control of isolated nervous tissue under optical access: Application to multi-electrode recordings
A system for precise temperature control of isolated nervous tissue under optical access: Application to multi-electrode recordings
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DOI：
10.1016/j.jneumeth.2013.06.007
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发表时间：
2013-09-30
影响因子：
3
通讯作者：
Ammermueller, Josef
中科院分区：
文献类型：
--
作者：
Ahlers, Malte T.;Ammermueller, Josef
背景：由于温度严重影响所有的生理过程，在电生理测量中精确的温度控制是必不可少的。然而，之前描述的回火系统方法中没有一种是完全令人满意的细胞外记录与尖锐的多电极阵列（MEAs）。新方法：我们开发了一种装置，为离体制备提供均匀回火和同时光透明的阶段。我们系统的基于珀尔帖元素的回火单元与制备阶段物理分离，避免了细胞外记录的电干扰。我们在微控制器上实现了一个数字反馈控制器，以尽量减少实际温度和设定点温度之间的偏差。结果：我们的回火系统允许在10℃至45℃范围内工作，稳态控制误差在0.052℃（RMSE）至0.115℃（RMSE）之间。为了证明我们系统的多功能性，我们在不同温度条件下对离体视网膜神经节细胞进行了细胞外MEA记录。我们发现即使很小的温度变化也会对光响应特性产生强烈的影响。与现有方法的比较：目前使用的加热系统允许顶部和底部光学进入制备通常表现出低温精度，精度或均匀性。结论：该系统不仅适用于生理温度条件下多种物种的实验，也适用于一般温度对生理影响的研究。虽然该装置是为视网膜的MEA记录而开发的，但在需要从顶部和底部同时进行光学访问的其他情况下，它可能是有用的。(C) 2013 Elsevier B.V.版权所有
Background: Since temperature severely affects all physiological processes, exact temperature control during electrophysiological measurements is indispensable. However, none of the tempering system approaches previously described is fully satisfactory for extracellular recordings with sharp multielectrode arrays (MEAs).New method: We developed a set-up offering a homogeneously tempered and at the same time light-transparent stage for an ex vivo preparation. The Peltier element based tempering unit of our system is physically separated from the preparation stage avoiding electrical disturbances of extracellular recordings. We implemented a digital feedback controller on a microcontroller to minimise the deviation between actual and set point temperature.Results: Our tempering system allows operation from 10 degrees C to 45 degrees C with a control error in steady state between 0.052 degrees C (RMSE) and 0.115 degrees C (RMSE). To document the versatility of our system, we performed extracellular MEA recordings from retinal ganglion cells of isolated retina under different temperature conditions. We found strong influences on light response properties, even for small temperature changes.Comparison with existing methods: Currently used heating systems that allow top and bottom side optical access to a preparation typically exhibit low temperature accuracy, precision or homogeneity.Conclusions: Our system is adequate not only for experiments on a variety of species under physiological temperature conditions but also for studies on temperature effects on physiology in general. Though the setup was developed for the context of MEA recordings from retina it may be useful in other cases where optical access to the preparation from both, top and bottom side is required. (C) 2013 Elsevier B.V. All rights reserved.