Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency.
Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency.
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DOI：
10.1016/j.biortech.2021.126484
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发表时间：
2021-12
影响因子：
11.4
通讯作者：
WEN-JIE Yu;Litao Zhang;Jing Zhao;Jianguo Liu
中科院分区：
文献类型：
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作者：
WEN-JIE Yu;Litao Zhang;Jing Zhao;Jianguo Liu
有效的诱导剂和胁迫条件在调节虾青素生物合成中起着至关重要的作用。本研究报道了一种结合外源添加草酰乙酸（OA）和氮缺乏促进雨生红球藻（Haematococcus pluvialis）生产虾青素的策略。显著地，在氮缺乏的第7天，添加10 mM-OA使细胞内的虾青素含量增加约7.18倍，同时虾青素酯的含量也随之增加。为了进一步阐明OA对虾青素合成的作用及其机制，本研究对外源OA处理雨生红球藻的生理代谢进行了分析。结果表明，外源OA对呼吸作用的促进作用大于光合作用。同时，Embden-Meyerhof-Parnas途径、磷酸戊糖途径和三羧酸循环中的代谢产物水平明显增加。呼吸代谢途径的增强导致底物水平的升高，从而直接促进了虾青素的合成。本研究结果为优化虾青素生产提供了一条新的有效途径。
Effective inducers and stress conditions play an essential role in the regulation of astaxanthin biosynthesis. This study reports a strategy developed by combining exogenous addition of oxaloacetate (OA) with nitrogen deficiency to facilitate astaxanthin production inHaematococcus pluvialis. Significantly, addition of 10 mM-OA enhanced the cellular astaxanthin content about 7.18-fold under nitrogen deficiency on day 7, with the content of astaxanthin esters increased concomitantly. To further elucidate the role and mechanism of OA on astaxanthin synthesis, the physiological and metabolic analyses ofH.pluvialistreated with exogenous OA were performed. The results showed that exogenous OA promoted respiration over photosynthesis. Concurrently, the metabolite levels in the Embden-Meyerhof-Parnas pathway, pentose phosphate pathway and tricarboxylic acid cycle obviously increased. The enhancement of respiratory metabolic pathways led to elevated levels of substrates, thus directly promoted astaxanthin synthesis. The present findings provide a new and effective approach for optimizing astaxanthin production.