Delayed larval development in Anopheles mosquitoes deprived of Asaia bacterial symbionts.
Delayed larval development in Anopheles mosquitoes deprived of Asaia bacterial symbionts.
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DOI：
10.1186/1471-2180-12-s1-s2
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发表时间：
2012-01-18
期刊：
影响因子：
4.2
通讯作者：
Favia G
中科院分区：
文献类型：
--
作者：
Chouaia B;Rossi P;Epis S;Mosca M;Ricci I;Damiani C;Ulissi U;Crotti E;Daffonchio D;Bandi C;Favia G
近年来，醋酸菌已被证明经常与昆虫相关，但对其在节肢动物宿主中的生物学作用的了解有限。 Asaia 属的醋酸菌是史氏按蚊微生物群的主要组成部分，这一发现使这种蚊子成为研究这一新型共生体的有用模型。在这里，我们提出的实验结果为 Asaia 在 An 中的有益作用提供了第一个证据。斯蒂芬西。 An 的幼虫。用对野生型 Asaia spp. 有效的抗生素利福平处理不同阶段的史蒂芬西氏幼虫，并评估其对幼虫发育的影响。用抗生素处理的幼虫表现出发育延迟和后期龄期出现的不同步。在用利福平处理但补充了利福平抗性 Asaia 突变株的幼虫中，幼虫发育与未暴露于抗生素的对照幼虫相当。对三个蚊子种群细菌多样性的分析证实，经过抗生素处理的幼虫中，Asaia 的水平大幅下降，因为通过 PCR-DGGE（变性梯度凝胶电泳）无法检测到共生体，而在饮食中添加利福平和抗生素抗性突变体的昆虫以及未暴露的昆虫中，始终发现 Asaia 存在。 到抗生素。本文报道的结果表明，Asaia 共生体在 An 的正常发育中发挥着有益的作用。史蒂芬西幼虫。
In recent years, acetic acid bacteria have been shown to be frequently associated with insects, but knowledge on their biological role in the arthropod host is limited. The discovery that acetic acid bacteria of the genus Asaia are a main component of the microbiota of Anopheles stephensi makes this mosquito a useful model for studies on this novel group of symbionts. Here we present experimental results that provide a first evidence for a beneficial role of Asaia in An. stephensi. Larvae of An. stephensi at different stages were treated with rifampicin, an antibiotic effective on wild-type Asaia spp., and the effects on the larval development were evaluated. Larvae treated with the antibiotic showed a delay in the development and an asynchrony in the appearance of later instars. In larvae treated with rifampicin, but supplemented with a rifampicin-resistant mutant strain of Asaia, larval development was comparable to that of control larvae not exposed to the antibiotic. Analysis of the bacterial diversity of the three mosquito populations confirmed that the level of Asaia was strongly decreased in the antibiotic-treated larvae, since the symbiont was not detectable by PCR-DGGE (denaturing gradient gel electrophoresis), while Asaia was consistently found in insects supplemented with rifampicin plus the antibiotic-resistant mutant in the diet, and in those not exposed to the antibiotic. The results here reported indicate that Asaia symbionts play a beneficial role in the normal development of An. stephensi larvae.