Next-Generation Lineage Tracing and Fate Mapping to Interrogate Development.
Next-Generation Lineage Tracing and Fate Mapping to Interrogate Development.
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DOI：
10.1016/j.devcel.2020.10.021
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发表时间：
2020-11
影响因子：
11.8
通讯作者：
S. VanHorn;Samantha A. Morris
中科院分区：
文献类型：
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作者：
S. VanHorn;Samantha A. Morris
血统追踪和命运图谱是追踪细胞及其后代的重叠但不同的学科，在过去的一个世纪里发展迅速。谱系追踪的目的是识别从单个细胞产生的所有后代，将他们置于谱系层次中。最近出现的基因组技术，如单细胞和空间转录，促进了复杂的新方法，以重建高分辨率的血统关系。相比之下，自20世纪70年代以来，命运图一直保持相对静止的状态。命运图显示了胚胎的哪些部分将发育成哪些组织。然而，命运地图提供了空间信息，这些信息往往在谱系重建中丢失，可以提供对发展的基本机械洞察。在这里，我们广泛地回顾了命运地图和血统追踪方法的起源。我们专注于单细胞基因组学进步所允许的谱系追踪方面的最新发展。最后，我们探索了利用这些新技术来合成高分辨率命运地图的当前潜力，并讨论了它们在新深度询问发展的潜力。
Lineage tracing and fate mapping, overlapping yet distinct disciplines to follow cells and their progeny, have evolved rapidly over the last century. Lineage tracing aims to identify all progeny arising from an individual cell, placing them within a lineage hierarchy. The recent emergence of genomic technologies, such as single-cell and spatial transcriptomics, has fostered sophisticated new methods to reconstruct lineage relationships at high resolution. In contrast, fate maps, schematics showing which parts of the embryo will develop into which tissue, have remained relatively static since the 1970s. However, fate maps provide spatial information, often lost in lineage reconstruction, that can offer fundamental mechanistic insight into development. Here, we broadly review the origins of fate mapping and lineage tracing approaches. We focus on the most recent developments in lineage tracing, permitted by advances in single-cell genomics. Finally, we explore the current potential to leverage these new technologies to synthesize high-resolution fate maps and discuss their potential for interrogating development at new depths.