A self-contained, programmable microfluidic cell culture system with real-time microscopy access
A self-contained, programmable microfluidic cell culture system with real-time microscopy access
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DOI：
10.1007/s10544-011-9615-6
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发表时间：
2012-04-01
影响因子：
2.8
通讯作者：
Dufva, Martin
中科院分区：
文献类型：
--
作者：
Skafte-Pedersen, Peder;Hemmingsen, Mette;Dufva, Martin
利用微流体技术是提高细胞生物学研究的通量和自动化程度的一种很有前途的方法。我们提出了一个完整的独立系统，用于自动细胞培养和实时光学读出实验。该系统提供了高度的用户友好性，由于简单的结构原则和紧凑的集成标准仪器的稳定性。此外，独立的系统是高度便携的，可以在工作站之间转移，如层流工作台，孵化器和显微镜。容纳24个单独的入口通道，使系统能够在单个芯片上执行并行，可编程和多条件分析。模块化方法提供了系统的多功能性，并允许根据应用使用许多不同的芯片。我们通过演示片上无源开关和蠕动驱动流的混合来验证系统的性能。芯片上的细胞培养，包括芯片上的转染和暂时可编程的基因表达，证明了生物测定的适用性。
Utilizing microfluidics is a promising way for increasing the throughput and automation of cell biology research. We present a complete self-contained system for automated cell culture and experiments with real-time optical read-out. The system offers a high degree of user-friendliness, stability due to simple construction principles and compactness for integration with standard instruments. Furthermore, the self-contained system is highly portable enabling transfer between work stations such as laminar flow benches, incubators and microscopes. Accommodation of 24 individual inlet channels enables the system to perform parallel, programmable and multiconditional assays on a single chip. A modular approach provides system versatility and allows many different chips to be used dependent upon application. We validate the system's performance by demonstrating on-chip passive switching and mixing by peristaltically driven flows. Applicability for biological assays is demonstrated by on-chip cell culture including on-chip transfection and temporally programmable gene expression.