The genetic basis of grain protein content in rice by genome-wide association analysis
The genetic basis of grain protein content in rice by genome-wide association analysis
复制标题
全基因组关联分析水稻籽粒蛋白质含量的遗传基础
DOI：
10.1007/s11032-022-01347-z
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发表时间：
2023-01-01
影响因子：
3.1
通讯作者：
He, Yuqing
中科院分区：
文献类型：
--
作者：
Chen, Pingli;Lou, Guangming;He, Yuqing
稻米蛋白质含量是决定稻米营养品质、蒸煮品质和食用品质的重要因素。迄今为止，虽然已经在水稻中鉴定了许多影响GPC的基因，但大多数基因都是利用突变体克隆的，只有少数基因在自然群体中被克隆。在这项研究中，在全基因组关联研究（GWAS）中检测到135个显著位点，其中许多位点可以在不同年份和人群中重复检测。在近等基因系F2群体（NIL-F2）中进一步鉴定和验证了4个影响水稻GPC的微效数量性状位点qPC2.1、qPC7.1、qPC7.2和qPC1.1，分别解释了9.82%、43.4%、29.2%和13.6%的表型变异。用敲低突变体评估了相关flo 5的作用，这些突变体表现出增加的籽粒垩白率和GPC。利用单倍型和表达谱分析了显著关联位点区域的三个候选基因。本研究结果将有助于通过GPC基因的克隆阐明水稻蛋白质合成和积累的遗传调控网络，并为水稻品质遗传改良中的标记辅助选择提供新的显性等位基因。
The grain protein content (GPC) of rice is an important factor that determines its nutritional, cooking, and eating qualities. To date, although a number of genes affecting GPC have been identified in rice, most of them have been cloned using mutants, and only a few genes have been cloned in the natural population. In this study, 135 significant loci were detected in a genome-wide association study (GWAS), many of which could be repeatedly detected across different years and populations. Four minor quantitative trait loci affecting rice GPC at four significant association loci,qPC2.1,qPC7.1,qPC7.2, andqPC1.1, were further identified and validated in near-isogenic line F2populations (NIL-F2), explaining 9.82, 43.4, 29.2, and 13.6% of the phenotypic variation, respectively. The role of the associatedflo5was evaluated with knockdown mutants, which exhibited both increased grain chalkiness rate and GPC. Three candidate genes in a significant association locus region were analyzed using haplotype and expression profiles. The findings of this study will help elucidate the genetic regulatory network of protein synthesis and accumulation in rice through cloning of GPC genes and provide new insights on dominant alleles for marker-assisted selection in the genetic improvement of rice grain quality.