ANALYSIS OF GENOMIC AND INTRACELLULAR VIRAL RNAS OF SMALL PLAQUE MUTANTS OF MOUSE HEPATITIS-VIRUS, JHM STRAIN
ANALYSIS OF GENOMIC AND INTRACELLULAR VIRAL RNAS OF SMALL PLAQUE MUTANTS OF MOUSE HEPATITIS-VIRUS, JHM STRAIN
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DOI：
10.1016/0042-6822(84)90335-0
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发表时间：
1984-01-01
期刊：
影响因子：
3.7
通讯作者：
FUJIWARA, K
中科院分区：
文献类型：
--
作者：
MAKINO, S;TAGUCHI, F;FUJIWARA, K
通过 T1 抗性寡核苷酸指纹图谱分析从持续感染培养物 (JHM-CC) 中分离出的小鼠肝炎病毒株 JHM (MHV-JHM) 和 2 个噬菌斑突变体（1a 和 2c）的基因组 RNA 和细胞内 RNA。还分析了同一持续感染的不同传代水平释放的病毒群体（JHM-CC病毒）的基因组RNA。分析显示了 > 45 个 T1 抗性寡核苷酸在基因组和细胞内 RNA 中的位置，并证实了早期研究表明 MHV-JHM 的 6 个亚基因组 RNA 形成 3'' 共端嵌套组，在 5'' 方向延伸不同长度。该分析还在每个亚基因组 RNA 中鉴定出在 mRNA 合成过程中源自基因组非连续区域的那些大 T1 寡核苷酸。从突变病毒的分析中可以得出两个重要结论。首先，从持续感染中释放的病毒种群代表了相当稳定的病毒混合物，并且不会出现作为培养物中主要物种的变异体的波动出现。其次，对于特定的突变病毒胞内RNA，亚基因组mRNA合成过程中发生的序列重排与野生型病毒相应胞内RNA中的序列重排不同。结果可能表明领导者/身体融合过程中存在以前未被认识到的潜在灵活性。
The genomic RNA and intracellular RNA of mouse hepatitis virus strain JHM (MHV-JHM) and 2 plaque mutants (1a and 2c), which were isolated from a persistently infected culture (JHM-CC), were analyzed by T1-resistant oligonucleotide fingerprinting. The genomic RNA of the virus population (JHM-CC virus) released from different passage levels of the same persistent infection was also analyzed. The analysis shows the locations within the genomic and intracellular RNA of > 45 T1-resistant oligonucleotides and confirm earlier studies showing that the 6 subgenomic RNA of MHV-JHM form a 3'' coterminal nested set which extends for different lengths in a 5'' direction. The analysis also identifies in each subgenomic RNA those large T1 oligonucleotides derived from noncontiguous regions of the genome during mRNA synthesis. Two important conclusions can be reached from analysis of the mutant viruses. First, the virus population released from the persistent infection represents a fairly constant mixture of viruses, and the fluctuating emergence of variants as predominant species in the culture does not occur. Second, for particular intracellular RNA of mutant viruses, the sequence rearrangements occurring during subgenomic mRNA synthesis are different from those in the corresponding intracellular RNA of wild-type virus. The result may indicate a potential flexibility in the leader/body fusion process that has not been previously recognized.