A novel DSF-like signal from Burkholderia cenocepacia interferes with Candida albicans morphological transition
A novel DSF-like signal from Burkholderia cenocepacia interferes with Candida albicans morphological transition
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DOI：
10.1038/ismej.2007.76
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发表时间：
2008-01-01
期刊：
影响因子：
11
通讯作者：
Zhang, Lian-Hui
中科院分区：
文献类型：
--
作者：
Boon, Calvin;Deng, Yinyue;Zhang, Lian-Hui
除了产生致命的抗生素外，微生物还可能使用一种新形式的拮抗机制，其中信号分子被输出以影响基因表达，从而影响其竞争对手的生态能力。我们在这里报告的分离和表征的一种新的信号分子，顺式-2-十二碳烯酸（BDSF），从伯克霍尔德氏菌cenocepacia。BDSF在结构上类似于由野油菜黄单胞菌的RpfF酶产生的扩散信号因子（DSF）。缺失分析表明，Bcam0581，它编码的RpfF同源，是必不可少的BDSF生产。该基因在洋葱伯克霍尔德菌复合体中高度保守且广泛存在。外源添加BDSF恢复生物膜和胞外多糖生产表型的野油菜黄单胞菌致病变种。campestris DSF缺陷突变体，突出了其在物种间信号传导中的潜在作用。进一步的分析表明，白色念珠菌芽管的形成强烈抑制与B的共培养。Bcam0581的缺失或通过外源添加生理相关水平的BDSF而消除细菌病原体的抑制能力。作为B。cenocepacia和C.白念珠菌是常见的人类病原体，因此BDSF信号及其活性的鉴定提供了对其拮抗相互作用的分子基础的新认识，其对微生物生态学和发病机制的重要性现在变得显而易见。
In addition to producing lethal antibiotics, microorganisms may also use a new form of antagonistic mechanism in which signal molecules are exported to influence the gene expression and hence the ecological competence of their competitors. We report here the isolation and characterization of a novel signaling molecule, cis-2-dodecenoic acid (BDSF), from Burkholderia cenocepacia. BDSF is structurally similar to the diffusible signal factor (DSF) that is produced by the RpfF enzyme of Xanthomonas campestris. Deletion analysis demonstrated that Bcam0581, which encodes an RpfF homologue, was essential for BDSF production. The gene is highly conserved and widespread in the Burkholderia cepacia complex. Exogenous addition of BDSF restored the biofilm and extracellular polysaccharide production phenotypes of Xanthomonas campestris pv. campestris DSF-deficient mutants, highlighting its potential role in inter-species signaling. Further analyses showed that Candida albicans germ tube formation was strongly inhibited by either coculture with B. cenocepacia or by exogenous addition of physiological relevant levels of BDSF, whereas deletion of Bcam0581 abrogated the inhibitory ability of the bacterial pathogen. As B. cenocepacia and C. albicans are frequently encountered human pathogens, identification of the BDSF signal and its activity thus provides a new insight into the molecular grounds of their antagonistic interactions whose importance to microbial ecology and pathogenesis is now becoming evident.