Flow-Responsive Vascular Endothelial Growth Factor Receptor-Protein Kinase C Isoform Epsilon Signaling Mediates Glycolytic Metabolites for Vascular Repair
Flow-Responsive Vascular Endothelial Growth Factor Receptor-Protein Kinase C Isoform Epsilon Signaling Mediates Glycolytic Metabolites for Vascular Repair
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DOI：
10.1089/ars.2017.7044
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发表时间：
2018-01-01
影响因子：
6.6
通讯作者：
Hsiai, Tzung K.
中科院分区：
文献类型：
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作者：
Baek, Kyung In;Li, Rongsong;Hsiai, Tzung K.
目的：血流动力学切应力参与维持血管氧化还原状态。阐明血流介导的内皮代谢物使我们能够发现代谢生物标志物和治疗靶点。我们假设，血流响应性血管内皮生长因子受体（VEGFR）-蛋白激酶C亚型（PKC）-6-磷酸果糖-2-激酶/果糖-2，6-二磷酸酶3（PFKFB 3）信号转导调节糖酵解代谢产物用于血管修复。结果如下：在人主动脉内皮细胞中，双向振荡流（振荡剪切应力[OSS]：0.13 dynecm（-2），1Hz）比单向脉动流（脉动剪切应力[PSS]：23 +/- 8 dynecm（-2），1Hz）更大程度地上调VEGF依赖性PKC表达（p
Aims: Hemodynamic shear stress participates in maintaining vascular redox status. Elucidating flow-mediated endothelial metabolites enables us to discover metabolic biomarkers and therapeutic targets. We posited that flow-responsive vascular endothelial growth factor receptor (VEGFR)-protein kinase C isoform epsilon (PKC)-6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) signaling modulates glycolytic metabolites for vascular repair. Results: Bidirectional oscillatory flow (oscillatory shear stress [OSS]: 0.13 dynecm(-2) at 1Hz) upregulated VEGFR-dependent PKC expression to a greater degree than did unidirectional pulsatile flow (pulsatile shear stress [PSS]: 23 +/- 8 dynecm(-2) at 1Hz) in human aortic endothelial cells (p