Plasmodium Vivax Malarial Paroxysms
Plasmodium Vivax Malarial Paroxysms
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间日疟原虫疟疾发作
DOI：
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发表时间：
1994
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影响因子：
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通讯作者：
K. Mendis
中科院分区：
文献类型：
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作者：
M. Kanthi Perera;R. Carter;Renu Goonewardene;K. Mendis;R. Carter;G. E. Grau;K. Mendis
本文检测了间日疟病人和恢复期病人外周血单个核细胞（PBMC）中CD 3 ~+细胞百分率及α/~和β/~ 8 T细胞受体（TCR）。用单克隆抗体标记细胞，用荧光素或藻红蛋白染色，并通过紫外（UV）显微镜检查。与非疟疾对照相比，在临床发作期间，在非免疫间日疟原虫患者中观察到3//8 T细胞的比例和绝对数量的高度显著增加（分别为p <0.005和p <0.001，Student’s t检验）。这些T细胞通常不超过PBMC的3-5%，在这些临床症状严重的非免疫患者中，在发作期间占PBMC的~<30%。在感染期间、发作期间和恢复期，在这些非免疫患者中也观察到3//8 T细胞的不太显著的增加。相比之下，在一组年龄匹配的半免疫患者居住在该国的疟疾流行地区，其中临床疾病相对较轻，有没有增加3//8 T细胞无论是在感染期间，甚至在发作，或恢复。通过临床评分测量的患者疾病症状的严重程度与外周血中3//8 T细胞的比例呈正相关（r = 0.53，p <0.01），胃肠道症状、恶心、厌食和呕吐的患病率和严重程度与3//8 T细胞比例之间的相关性最显著（r = 0.49，p = 0.002）。这些发现表明，3//8 T细胞在疟疾的发病机制中发挥作用，可能在一般的体质紊乱中，特别是在疟疾的胃肠道病理学中。T淋巴细胞表达CD 3标志物，缺乏CD 4和CD 8标志物，已显示缺乏α和β基因的成熟信使（m）RNA，表达γ和β基因和蛋白质（1）。这种表达TCR-3//6的淋巴细胞构成成人外周血中的次要亚群，并且它们中的许多存在于脾脏中，并且在较小程度上存在于胸腺、扁桃体（2）以及大肠（3）和肺（4）的上皮中。携带TCR-3//8的细胞的生物学作用尚不清楚，但有迹象表明一些3//8 T细胞可介导细胞毒性（4）。3 ′/8 T细胞与c~/B T细胞相似，分泌淋巴因子IL-2、IFN-γ、TNF-α和TNF-β，表达细胞毒活性。
The percentage of peripheral blood mononuclear cells (PBMC) bearing the CD3 § phenotype and the ot/~ and 3//8 T cell receptors (TCR) in PBMC were examined in Plasmodium vivax malaria patients and convalescents. The cells were labeled with monoclonal antibodies, stained with either fluorescene or phycoerythrin, and examined by ultraviolet (UV) microscopy. A highly significant increase in both the proportion and the absolute numbers of 3//8 T cells (p <0.005 and <0.001, respectively, Student's t test) was observed in nonimmune P. vivax patients during clinical paroxysms compared to nonmalarial controls. These T cells, which normally constitute not more than 3-5% of PBMC, constituted ~<30% of PBMC during paroxysms in these nonimmune patients in whom the clinical symptoms were severe. A less significant increase of 3//8 T cells were also observed in these nonimmune patients during infection, between paroxysms and during convalescence. In contrast, in an age-matched group of semi-immune patients resident in a malaria-endemic region of the country, in whom the clinical disease was comparatively mild, there was no increase in 3//8 T cells either during infection, even during paroxysms, or convalescence. The severity of disease symptoms in patients as measured by a clinical score correlated positively with the proportion of 3//8 T cells in peripheral blood (r = 0.53, p <0.01), the most significant correlation being found between the prevalence and severity of gastrointestinal symptoms, nausea, anorexia, and vomiting, and the proportion of 3//8 T cells (r = 0.49, p = 0.002). These findings suggest that 3//8 T cells have a role to play in the pathogenesis of malaria, possibly in the general constitutional disturbances and particularly in gastrointestinal pathology in malaria. T lymphocytes, which express the CD3 marker and lack CD4 and CD8 markers, have been shown to lack mature messenger (m)RNA for ot and/3 genes and express the y and 8 genes and proteins (1). Such lymphocytes expressing the TCR-3//6 constitute a minor subpopulation in the peripheral blood of adults, and many of them reside in the spleen, and to a lesser extent in the thymus, tonsils (2), and the epithelia of the large intestine (3) and lung (4). The biological role of the cells bearing the TCR-3,/8 is as yet unclear, but there are indications that some 3//8 T cells can mediate cytotoxicity (4). 3'/8 T cells behave like c~/B T cells in that they secrete the lymphokines IL-2, IFN-y, TNF-o~ and ~3, and express cytotoxic …