PEGylated graphene oxide-mediated quercetin-modified collagen hybrid scaffold for enhancement of MSCs differentiation potential and diabetic wound healing
PEGylated graphene oxide-mediated quercetin-modified collagen hybrid scaffold for enhancement of MSCs differentiation potential and diabetic wound healing
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DOI：
10.1039/c8nr02538j
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发表时间：
2018-05-28
期刊：
影响因子：
6.7
通讯作者：
Liu, Hanping
中科院分区：
文献类型：
--
作者：
Chu, Jing;Shi, Panpan;Liu, Hanping
基于聚乙二醇（PEG）化氧化石墨烯（GO-PEG）的纳米级给药由于其功能性表面改性、上级溶解性/生物相容性和可控的药物释放能力而在生物医学应用中受到关注。然而，在糖尿病中应用这些迷人的纳米材料时，受损的皮肤再生仍然有限，并且需要解决有关胶原蛋白增生不足和血液供应不足的关键问题。因此，具有生物相容性和生物可降解性的高性能组织工程支架对于糖尿病伤口愈合至关重要。天然和人工脱细胞真皮基质（ADM）支架与空间组织胶原纤维可以提供一个合适的架构和环境，细胞附着和增殖。本研究利用GO-PEG介导的槲皮素（GO-PEG/Que）修饰ADM（ADM-GO-PEG/Que）构建了一种新型胶原-纳米材料-药物杂化支架。该复合支架具有生物相容性好、细胞粘附性强、可促进间充质干细胞（MSC）的附着和增殖、槲皮素的传导电位具有良好的上级稳定性和可调节性，可诱导MSC分化为脂肪细胞和成骨细胞;以及用于促进糖尿病伤口修复中的胶原沉积和血管生成的可生物降解的生物相容性界面。这项研究为设计创新的GO-PEG基胶原蛋白杂化支架提供了新的前景，可用于有效的治疗药物递送，干细胞治疗，组织工程和再生医学。
Nanoscale delivery based on polyethylene glycol (PEG) ylated graphene oxide (GO-PEG) merits attention for biomedical applications owing to its functional surface modification, superior solubility/biocompatibility and controllable drug release capability. However, impaired skin regeneration in applications of these fascinating nanomaterials in diabetes is still limited, and critical issues need to be addressed regarding insufficient collagen hyperplasia and inadequate blood supply. Therefore, a high-performance tissue engineering scaffold with biocompatible and biodegradable properties is essential for diabetic wound healing. Natural and artificial acellular dermal matrix (ADM) scaffolds with spatially organized collagen fibers can provide a suitable architecture and environment for cell attachment and proliferation. Here, a novel collagen-nanomaterial-drug hybrid scaffold was constructed from GO-PEG-mediated quercetin (GO-PEG/Que)-modified ADM (ADM-GO-PEG/Que). The resulting unique and versatile hybrid scaffold exhibited multiple advantages, including the following: a biocompatible, cell-adhesive surface for accelerating mesenchymal stem cell (MSC) attachment and proliferation; superior stability and adjustability of the conduction potential of quercetin for inducing the differentiation of MSCs into adipocytes and osteoblasts; and a biodegradable nanofiber interface for promoting collagen deposition and angiogenesis in diabetic wound repair. This study provides new prospects for the design of innovative GO-PEG-based collagen hybrid scaffolds for application in efficient therapeutic drug delivery, stem cell-based therapies, tissue engineering and regenerative medicine.