Biological effect of anti-fucosyl GM1 ganglioside antibody on cyclic adenosine 3',5'-monophosphate production in FRTL5 rat thyroid cells.
Biological effect of anti-fucosyl GM1 ganglioside antibody on cyclic adenosine 3',5'-monophosphate production in FRTL5 rat thyroid cells.
复制标题
抗岩藻糖基 GM1 神经节苷脂抗体对 FRTL5 大鼠甲状腺细胞中环腺苷 3,5-单磷酸产生的生物学效应。
DOI：
10.1016/0006-291x(87)90327-5
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
H. Imura
中科院分区：
文献类型：
--
作者：
S. Kosugi;T. Akamizu;M. Miyamoto;H. Sugawa;M. Iwamori;Y. Nagai;T. Mori;H. Imura
在培养的FRTL 5大鼠甲状腺细胞中，添加在兔中产生的特异性抗岩藻糖基GM1抗体导致内源性和促甲状腺激素（TSH）或甲状腺刺激抗体刺激的环腺苷酸（cAMP）产生的剂量依赖性抑制。此外，抗体抑制由前列腺素El和毛喉素诱导的cAMP增加。然而，抗岩藻糖基GM1抗体不影响[125 I]牛TSH与溶解的猪甲状腺TSH受体或FRTL 5细胞的结合。总之，岩藻糖基GM1是甲状腺细胞的特定膜成分之一，似乎参与腺苷酸环化酶刺激或cAMP生成。此外，神经节苷脂的生物学效应似乎不通过TSH受体介导，表明受体后机制。
Addition of specific anti-fucosyl GM1 antibody raised in a rabbit caused dose-dependent inhibition of endogenous and thyrotropin (TSH)- or thyroid stimulating antibody-stimulated cyclic adenosine 3′,5′-monophosphate (cAMP) production in cultured FRTL5 rat thyroid cells. Further, the antibody inhibited the cAMP increase induced by prostaglandin El and forskolin. However, anti-fucosyl GM1 antibody did not affect the binding of [125I]bovine TSH to solubilized porcine thyroid TSH receptor or to FRTL5 cells. In conclusion, fucosyl GM1 is one of the specific membrane components of thyrocytes and appears to be involved in adenylate cyclase stimulation or cAMP generation. Further, the biological effects of the ganglioside do not seem to be mediated by the TSH receptor, suggesting a post receptor mechanism.