Salinity-dominated change in community structure and ecological function of Archaea from the lower Pearl River to coastal South China Sea
Salinity-dominated change in community structure and ecological function of Archaea from the lower Pearl River to coastal South China Sea
复制标题
DOI：
10.1007/s00253-014-5838-9
复制
发表时间：
2014-06
影响因子：
5
通讯作者：
W. Xie;Chuanlun Zhang;Xuedan Zhou;Peng Wang
中科院分区：
文献类型：
--
作者：
W. Xie;Chuanlun Zhang;Xuedan Zhou;Peng Wang
海洋在全球地球化学循环中具有多重作用。然而，我们对环境因素如何影响不同古菌谱系的多样性和功能的了解仍然有限。研究珠江下游（主要是北江支流）、珠江口和南海沿岸沉积物中古菌的丰度和群落结构的变化，以探讨古菌生态功能沿着盐度梯度的变化。对万山群岛北江支流飞来峡水库沉积物样品进行了16 SrDNA基因的焦磷酸测序，其盐度范围为0.1 ~ 31.2‰。与耐盐性的栽培代表，甲烷菌属甲烷调节菌，甲烷八叠球菌属和亚硝化球菌属内Thaumarchaeota的氨氧化菌（AOA）是丰富的淡水沉积物中的北江支流，而海洋相关的属甲烷球菌和亚硝化菌是最丰富的甲烷菌和AOA，分别在河口和沿海的南海。然而，总的产甲烷菌的百分比下降和Thaumarchaeota增加，分别与盐度。Crenarchaeota门主要是由类级谱系，没有培养的代表，这是集体更丰富的河口和沿海的南海相比，淡水网站。研究表明，盐度是影响珠江北江支流及其河口和南海沿岸古菌群落结构和生态功能的主导因子，这与世界其他地区盐度对微生物多样性的控制是一致的。
Archaea have multiple roles in global biogeochemical cycles. However, we still have limited knowledge about how environmental factors affect the diversity and function of different archaeal lineages. The goal of this study was to examine the change in the abundance and community structure of Archaea in the sediments collected from the lower Pearl River (mainly North River tributary), its estuary, and coastal South China Sea (SCS) in order to evaluate how archaeal ecological function might change along the salinity gradient. Pyrosequencing of the 16S rDNA gene of Archaea was performed on sediment samples from Feilaixia Dam on the North River tributary to Wanshan islands, which have a salinity range of 0.1 to 31.2‰. Consistent with the salt tolerance of cultivated representatives, methanogens in the genera Methanoregula, Methanosaeta, and Methanosarcina and Nitrososphaera within Thaumarchaeota of the ammonia-oxidizing Archaea (AOA) were abundant in freshwater sediments of the North River tributary, whereas the marine-associated genera Methanococcoides and Nitrosopumilus were the most abundant methanogens and AOA, respectively, in the estuary and coastal SCS. However, the percentages of total methanogens decreased and Thaumarchaeota increased with salinity, respectively. The phylum Crenarchaeota was largely represented by class-level lineages with no cultivated representatives, which collectively were more abundant in the estuary and coastal SCS in comparison to freshwater sites. This study indicates that salinity is the dominating factor affecting archaeal community structure and ecological function from the North River tributary of the Pearl River, its estuary, and coastal SCS, which is consistent with salinity control on microbial diversity in other regions of the world.