MITOCHONDRIAL TRANSFORMATION IN YEAST BY BOMBARDMENT WITH MICROPROJECTILES
MITOCHONDRIAL TRANSFORMATION IN YEAST BY BOMBARDMENT WITH MICROPROJECTILES
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DOI：
10.1126/science.2836954
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发表时间：
1988-06-10
期刊：
影响因子：
56.9
通讯作者：
BUTOW, RA
中科院分区：
文献类型：
--
作者：
JOHNSTON, SA;ANZIANO, PQ;BUTOW, RA
线粒体和叶绿体的遗传转化一直是一个棘手的问题。新开发的“生物射弹”（生物弹道）过程用于将DNA递送到酵母细胞中以稳定地转化其线粒体。一个nonreverting菌株，这是呼吸缺陷，因为在线粒体axi3基因的缺失，轰击钨微粒涂有DNA轴承序列，可以纠正axi3缺失。获得呼吸感受态转化体，其中引入的axi3 DNA整合在线粒体基因组中的同源位点。细胞器基因组现在可以通过分子遗传技术以与核基因组相同的方式进行操纵。
The genetic transformation of mitochondria and chloroplasts has been an intractable problem. The newly developed "biolistic" (biological ballistic) process was used to deliver DNA into yeast cells to stably transform their mitochondria. A nonreverting strain, which is respiratory deficient because of a deletion in the mitochondrial axi3 gene, was bombarded with tungsten microprojectiles coated with DNA bearing sequences that could correct the axi3 deletion. Respiratory-competent transformants were obtained in which the introduced axi3 DNA is integrated at the homologous site in the mitochondrial genome. Organelle genomes can now be manipulated by molecular genetic techniques in the same way as nuclear genomes.