Sexual dimorphism in the content of progesterone and estrogen receptors, and their cofactors in the lung of adult rats
Sexual dimorphism in the content of progesterone and estrogen receptors, and their cofactors in the lung of adult rats
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DOI：
10.1016/j.steroids.2004.03.004
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发表时间：
2004-05-01
期刊：
影响因子：
2.7
通讯作者：
Camacho-Arroyo, I
中科院分区：
文献类型：
--
作者：
González-Arenas, A;Neri-Gómez, T;Camacho-Arroyo, I
孕酮和雌二醇在调节肺功能如通气和血管收缩中起重要作用。孕酮和雌二醇的基因组作用由它们的核受体介导：孕酮受体（PR）和雌激素受体（ER）。这些作用与类固醇受体和一些辅助因子之间的相互作用有关，这些辅助因子起辅助激活剂或辅助抑制剂的作用。在这项工作中，我们确定的内容PR亚型，ER-β，一个辅激活因子（SRC-1），一个辅阻遏物（SMRT）在两个雌性大鼠在发情周期和完整的男性的肺蛋白质印迹。在动情周期中，大鼠肺组织中PR-A的含量高于PR-B。两种PR亚型的含量在发情前期最高，而在发情期最低。而ER-β在发情前期含量最低，在发情期含量升高。SRC-I和SMRT的含量在发情间期增加。SRC-I含量在发情前期和发情期下降，SMRT含量在发情前期下降，在发情期再次上升。在成年雄性大鼠的肺中，PR亚型、ER-β和SMRT的含量低于雌性大鼠，而SRC-I的含量在两种性别中相似。我们的研究结果表明，在大鼠肺中的PR，ER-p和SMRT的含量以及它们的含量的生理水平的孕酮和雌二醇的关系的性别二型性。(C)2004年爱思唯尔公司All rights reserved.
Progesterone and estradiol play an important role in the regulation of lung functions such as ventilation and vasoconstriction. The genomic actions of progesterone and estradiol are mediated by their nuclear receptors: progesterone receptors (PR) and estrogen receptors (ER). These actions are linked to interactions between steroid receptors and some cofactors that function as coactivators or corepressors. In this work we determined the content of PR isoforms, ER-beta, one coactivator (SRC-1), and one corepressor (SMRT) in the lung of both female rats during the estrous cycle and intact males by Western blot. The rat lung presented a higher content of PR-A than that of PR-B during the estrous cycle. The highest content of both PR isoforms was observed on the day of proestrus whereas the lowest one was found on the day of estrus. In contrast, the content of ER-p was the lowest on the day of proestrus and it increased at estrus. The content of SRC-I and SMRT increased on the day of diestrus. SRC-I content decreased at proestrus and estrus, while SMRT content decreased at proestrus and increased again at estrus. In the lung of adult male rats the content of PR isoforms, ER-beta and SMRT was lower than in that of females, whereas the content of SRC-I was similar in both sexes. Our results suggest a sexual dimorphism in the content of PR, ER-p, and SMRT in the rat lung as well as a relation of their content to the physiological levels of progesterone and estradiol. (C) 2004 Elsevier Inc. All rights reserved.